Polynucleotide Phosphorylase from a Cyanobacterium (Synechococcus sp.): Subunit Composition and Properties

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dc.identifier.uri http://dx.doi.org/10.15488/2464
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/2490
dc.contributor.author Nolden, W.-T.
dc.contributor.author Richter, G.
dc.date.accessioned 2017-11-24T11:46:31Z
dc.date.available 2017-11-24T11:46:31Z
dc.date.issued 1982
dc.identifier.citation Nolden, W.-T.; Richter, G.: Polynucleotide Phosphorylase from a Cyanobacterium (Synechococcus sp.): Subunit Composition and Properties. In: Zeitschrift für Naturforschung - Section C Journal of Biosciences 37 (1982), Nr. 7-8, S. 600-608. DOI: https://doi.org/10.1515/znc-1982-7-809
dc.description.abstract Polynucleotide phosphorylase from cells of the cyanobacterium Synechococcus sp. has been purified 1400-fold by an improved procedure. The enzyme purified to homogeneity and lacking nuclease, phosphatase and protease contaminations reveals a single band of ADP polymerizing activity upon polyacrylamide gel electrophoresis under nondenaturing conditions which corresponds to a molecular mass of about 275 000. The enzyme migrates as a single polypeptide of Mr≈70 000 when subjected to gel electrophoresis in the presence of dodecyl sulfate indicating a composition of α4 for the native enzyme molecule. The isoelectric point of the purified enzyme as determined by isoelectric focusing was found to be at 4.2±0.1. Polynucleotide phosphorylase of Synechococcus is preferentially activated by Mg2+; Kcl has a significant stimulatory effect. © 1982, Walter de Gruyter. All rights reserved. eng
dc.language.iso eng
dc.publisher Berlin : De Gruyter
dc.relation.ispartofseries Zeitschrift für Naturforschung - Section C Journal of Biosciences 37 (1982), Nr. 7-8
dc.rights CC BY-NC-ND 3.0
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject Affinity Chromatography eng
dc.subject Isoelectric Focusing eng
dc.subject Poly(A) Synthesis eng
dc.subject Polyacrylamide Gel Electrophoresis eng
dc.subject Tetrameric Structure eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Polynucleotide Phosphorylase from a Cyanobacterium (Synechococcus sp.): Subunit Composition and Properties
dc.type article
dc.type Text
dc.relation.essn 1865-7125
dc.relation.issn 0939-5075
dc.relation.doi https://doi.org/10.1515/znc-1982-7-809
dc.bibliographicCitation.issue 7-8
dc.bibliographicCitation.volume 37
dc.bibliographicCitation.firstPage 600
dc.bibliographicCitation.lastPage 608
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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