1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.)

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dc.identifier.uri http://dx.doi.org/10.15488/2370
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/2396
dc.contributor.author Latza, Stefan
dc.contributor.author Berger, Ralf Günter
dc.date.accessioned 2017-11-24T07:40:26Z
dc.date.available 2017-11-24T07:40:26Z
dc.date.issued 1997
dc.identifier.citation Latza, S.; Berger, R.G.: 1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.). In: Zeitschrift für Naturforschung - Section C Journal of Biosciences 52 (1997), Nr. 11-12, S. 747-755. DOI: https://doi.org/10.1515/znc-1997-11-1205
dc.description.abstract Methyl and ethyl cinnamate are aroma volatiles frequently occurring in fruits. Evidence was obtained that the enzymatic transfer of cinnamic acid to endogenous alcohols present in fruits (methanol, ethanol. 1-propanol) depended on energy-rich 1-O-glycosyl esters of cinnamic acid which served as acyl donor molecules. A putative l-O-trans-cinnamoyl-β-D-gluco-pyranose: alcohol cinnamoyltransferase from cape gooseberry (Physalis peruviana L.) was active towards l-O-trans-cinnamoyl-β-D-glucopyranose and l-O-trans-cinnamoyl-β-D-gentio-biose. The enzyme was purified 290-fold by a protocol including ammonium sulphate precipitation, solubilization by Triton X-100, gel permeation and affinity chromatography on conca-navalin A. The acidic glycoprotein (pI = 4.8) most probably is membrane bound. The distribution of alcohol cinnamoyltransferase activity in gel chromatography fractions suggests a native Mrof 75,000. For l-O-trans-cinnamoyl-β-D-glucopyranose, an apparent Kmof 69 was determined. At pH > 6.0, non-enzymatic transesterification superposes the enzymatic transformation. eng
dc.language.iso eng
dc.publisher Berlin : De Gruyter
dc.relation.ispartofseries Zeitschrift für Naturforschung - Section C Journal of Biosciences 52 (1997), Nr. 11-12
dc.rights CC BY-NC-ND 3.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject Aroma Ester eng
dc.subject Flavour Precursor eng
dc.subject Glycoconjugate eng
dc.subject Methyl Cinnamate eng
dc.subject Phenylpropanoid Metabolism eng
dc.subject Physalis peruviana eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title 1-O-trans-Cinnamoyl-β-ᴅ -glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.) eng
dc.type article
dc.type Text
dc.relation.essn 1865-7125
dc.relation.issn 0939-5075
dc.relation.doi https://doi.org/10.1515/znc-1997-11-1205
dc.bibliographicCitation.issue 11-12
dc.bibliographicCitation.volume 52
dc.bibliographicCitation.firstPage 747
dc.bibliographicCitation.lastPage 755
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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