dc.identifier.uri |
http://dx.doi.org/10.15488/2306 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/2332 |
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dc.contributor.author |
Poehnert, Daniel
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dc.contributor.author |
Abbas, Mahmoud
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dc.contributor.author |
Maegel, Lavinia
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dc.contributor.author |
Sambale, Franziska
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dc.contributor.author |
Lavrentieva, Antonina
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dc.contributor.author |
Kreipe, Hans-Heinrich
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dc.contributor.author |
Klempnauer, Jürgen
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dc.contributor.author |
Winny, Markus
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dc.date.accessioned |
2017-11-17T08:34:59Z |
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dc.date.available |
2017-11-17T08:34:59Z |
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dc.date.issued |
2015 |
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dc.identifier.citation |
Poehnert, D.; Abbas, M.; Maegel, L.; Sambale, F.; Lavrentieva, A. et al.: Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model. In: Journal of Biomaterials Applications 30 (2015), Nr. 4, S. 463-471. DOI: https://doi.org/10.1177/0885328215592257 |
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dc.description.abstract |
Purpose To evaluate in vitro cytotoxicity/biocompatibility as well as in vivo tolerability of the novel polysaccharide 4DryField® PH, certified for haemostasis and adhesion prevention. Methods In vitro cytotoxicity/viability testing according to ISO EN 10,993 using murine and human tumour cell lines incubated with 4DryField® PH (PlantTec Medical GmbH). Using a rat model the impact of 4DryField® PH on animals viability and in vivo effects were macro- and micropathologically assessed. Results In vitro testing revealed no cytotoxic effect of 4DryField® PH nor enhancement of viability to tumour cell lines. In vivo viability of rats was unimpaired by 4DryField® PH. Bodyweight loss in animals with abdominal injury plus treatment with 4DryField® PH was in the range of controls and less than in injured rats without treatment. At day 7 after surgery no formation of adhesions, neither macroscopic nor histological remnants nor signs of foreign body reaction were present in animals without injury. In animals with peritoneal injury and 4DryField® PH application, histopathological observation revealed minor residuals of polysaccharide in the depth of wound cavity embedded in a thickened subperitoneal layer; however, with a suggested intact neoperitoneum. The presence of mononuclear cells surrounding polysaccharide particles in varying states of degradation was observable as well. Conclusion 4DryField® PH is not cytotoxic and does not enhance viability of tumour cell lines. High dose of 4DryField® PH of 1.09 g/kg bodyweight is well tolerated and reduces weight loss in animals with peritoneal injury. The biocompatibility of 4DryField® PH can be rated as being excellent. © SAGE Publications. |
eng |
dc.language.iso |
eng |
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dc.publisher |
London : SAGE Publications Ltd. |
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dc.relation.ispartofseries |
Journal of Biomaterials Applications 30 (2015), Nr. 4 |
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dc.rights |
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
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dc.subject |
4DryField® PH |
eng |
dc.subject |
adhesion prevention agent |
eng |
dc.subject |
biocompatibility |
eng |
dc.subject |
cytotoxicity |
eng |
dc.subject |
haemostatic agent |
eng |
dc.subject |
polysaccharide |
eng |
dc.subject |
rat model |
eng |
dc.subject |
Adhesion |
eng |
dc.subject |
Animals |
eng |
dc.subject |
Biocompatibility |
eng |
dc.subject |
Biomedical equipment |
eng |
dc.subject |
Cell culture |
eng |
dc.subject |
Cells |
eng |
dc.subject |
Cytology |
eng |
dc.subject |
Cytotoxicity |
eng |
dc.subject |
Polysaccharides |
eng |
dc.subject |
Tumors |
eng |
dc.subject |
Abdominal injury |
eng |
dc.subject |
Adhesion preventions |
eng |
dc.subject |
Cytotoxic effects |
eng |
dc.subject |
Foreign body reactions |
eng |
dc.subject |
In-vitro testing |
eng |
dc.subject |
Medical Devices |
eng |
dc.subject |
Mononuclear cells |
eng |
dc.subject |
Rat model |
eng |
dc.subject |
Rats |
eng |
dc.subject |
polysaccharide |
eng |
dc.subject |
abdominal injury |
eng |
dc.subject |
animal cell |
eng |
dc.subject |
animal experiment |
eng |
dc.subject |
animal model |
eng |
dc.subject |
animal tissue |
eng |
dc.subject |
Article |
eng |
dc.subject |
biocompatibility |
eng |
dc.subject |
cell growth |
eng |
dc.subject |
cell viability |
eng |
dc.subject |
controlled study |
eng |
dc.subject |
cytotoxicity |
eng |
dc.subject |
degradation |
eng |
dc.subject |
hemostasis |
eng |
dc.subject |
histology |
eng |
dc.subject |
human |
eng |
dc.subject |
human cell |
eng |
dc.subject |
in vitro study |
eng |
dc.subject |
in vivo study |
eng |
dc.subject |
laparotomy |
eng |
dc.subject |
medical device |
eng |
dc.subject |
mononuclear cell |
eng |
dc.subject |
mouse |
eng |
dc.subject |
nonhuman |
eng |
dc.subject |
peritoneal injury |
eng |
dc.subject |
priority journal |
eng |
dc.subject |
rat |
eng |
dc.subject |
tissue adhesion |
eng |
dc.subject |
tumor cell line |
eng |
dc.subject |
weight reduction |
eng |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
ger |
dc.title |
Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model |
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dc.type |
Article |
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dc.type |
Text |
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dc.relation.issn |
0885-3282 |
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dc.relation.doi |
https://doi.org/10.1177/0885328215592257 |
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dc.bibliographicCitation.issue |
4 |
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dc.bibliographicCitation.volume |
30 |
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dc.bibliographicCitation.firstPage |
463 |
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dc.bibliographicCitation.lastPage |
471 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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