Metabolic engineering of apple by overexpression of the MdMyb10 gene

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dc.identifier.uri http://dx.doi.org/10.15488/1944
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1969
dc.contributor.author Rihani, Khaled A.L.
dc.contributor.author Jacobsen, Hans-Jörg
dc.contributor.author Hofmann, Thomas
dc.contributor.author Schwab, Wilfried
dc.contributor.author Hassan, Fathi
dc.date.accessioned 2017-09-22T11:47:57Z
dc.date.available 2017-09-22T11:47:57Z
dc.date.issued 2017
dc.identifier.citation Rihani, K.A.L.; Jacobsen, H.-J.; Hofmann, T.; Schwab, W.; Hassan, F.: Metabolic engineering of apple by overexpression of the MdMyb10 gene. In: Journal of Genetic Engineering and Biotechnology 15 (2017), Nr. 1, S. 263-273. DOI: https://doi.org/10.1016/j.jgeb.2017.01.001
dc.description.abstract Flavonoids are low-molecular-weight phenolic compounds that are widely distributed in the plant kingdom. They have different roles in plant resistance to biotic and abiotic stresses. The transcription factor gene MdMyb10 (Gene Bank: DQ267896) was introduced into two apple (Malus domestica Borkh.) cultivars i.e. ‘Holsteiner Cox (HC)’ and ‘Gala’ via Agrobacterium-mediated transformation. The regenerated shoots were selected on kanamycin containing media. The presence of additional MdMyb10 gene in putative shoots was confirmed by PCR, RT-PCR and Southern blotting. Expression level of introduced MdMyb10 gene was analyzed by quantitative real time PCR. The results confirmed a dramatic increase in overexpression of MdMyb10 in the transgenic plants, up to 1261 and 847-folds for cultivars Holsteiner Cox and Gala, respectively compared to non-transformed negative control plants. HPLC-MS was used to determine the levels of different flavonoid compounds in both non-transgenic and transgenic plants. In MdMyb10 ‘HC’ transgenic plants, some of the polyphenols analyzed were enhanced while others were reduced in comparison to their levels in the non-transgenic plants. On the other hand, all of the analyzed polyphenol classes were induced in MdMyb10 ‘Gala’ transgenic plants in comparison to their levels in the non-transgenic plants. In the present study, the flavonoid pathway was successfully modified in apple by overexpressing the MdMyb10 transcription factor to validate the hypothesis of increased effect on plant disease resistance. eng
dc.description.sponsorship Islamic Development Bank
dc.language.iso eng
dc.publisher Amsterdam : Elsevier
dc.relation.ispartofseries Journal of Genetic Engineering and Biotechnology 15 (2017), Nr. 1
dc.rights CC BY-NC-ND 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0
dc.subject Agrobacterium eng
dc.subject Apple eng
dc.subject Flavonoids eng
dc.subject MdMyb10 eng
dc.subject Metabolic engineering eng
dc.subject Over-expression eng
dc.subject Transcription factors eng
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title Metabolic engineering of apple by overexpression of the MdMyb10 gene
dc.type article
dc.type Text
dc.relation.issn 1687157X
dc.relation.doi https://doi.org/10.1016/j.jgeb.2017.01.001
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 15
dc.bibliographicCitation.date
dc.bibliographicCitation.firstPage 263
dc.bibliographicCitation.lastPage 273
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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