dc.identifier.uri |
http://dx.doi.org/10.15488/17262 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/17390 |
|
dc.contributor.author |
Tran, Thi Chi
|
|
dc.contributor.author |
Maiss, Edgar
|
|
dc.contributor.author |
Rose, Hanna
|
|
dc.date.accessioned |
2024-04-30T08:56:43Z |
|
dc.date.available |
2024-04-30T08:56:43Z |
|
dc.date.issued |
2023 |
|
dc.identifier.citation |
Tran, T.C.; Maiss, E.; Rose, H.: The complete genome sequence of Neckar River virus confirms it to be a distinct member of the genus Tombusvirus in the family Tombusviridae. In: Archives of Virology (Formerly: Archiv für die gesamte Virusforschung) 168 (2023), Nr. 12, 296. DOI: https://doi.org/10.1007/s00705-023-05918-z |
|
dc.description.abstract |
Neckar River virus (NRV), first isolated from a water sample of the Neckar River (Germany) in the 1980s, was serologically characterized as a novel tombusvirus. In this study, the complete genome sequence was determined, and an infectious full-length cDNA clone was constructed. The genome organization of NRV (DSMZ PV-0270) resembles that of tombusviruses. The genome consists of 4739 nucleotides and contains five open reading frames (ORFs) and one additional putative ORF (pX) in the 3’-terminal region. Phylogenetic analysis and sequence comparisons confirmed NRV to be a member of the species Tombusvirus neckarfluminis in the genus Tombusvirus. The infectious full-length cDNA clone was constructed using Gibson assembly and subsequent infection of Nicotiana benthamiana plants by Rhizobium radiobacter inoculation. The virus derived from the full-length cDNA clone caused symptoms resembling those caused by the wild-type virus, but slightly milder. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Wien ; New York, NY : Springer |
|
dc.relation.ispartofseries |
Archives of Virology (Formerly: Archiv für die gesamte Virusforschung) 168 (2023), Nr. 12 |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0 |
|
dc.subject |
DNA, Complementary |
eng |
dc.subject |
Genome, Viral |
eng |
dc.subject |
Open Reading Frames |
eng |
dc.subject |
Phylogeny |
eng |
dc.subject |
RNA, Viral |
eng |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
|
dc.title |
The complete genome sequence of Neckar River virus confirms it to be a distinct member of the genus Tombusvirus in the family Tombusviridae |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1432-8798 |
|
dc.relation.issn |
0304-8608 |
|
dc.relation.doi |
https://doi.org/10.1007/s00705-023-05918-z |
|
dc.bibliographicCitation.issue |
12 |
|
dc.bibliographicCitation.volume |
168 |
|
dc.bibliographicCitation.firstPage |
296 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|