Dichloroacetate affects proliferation but not apoptosis in canine mammary cell lines

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dc.identifier.uri http://dx.doi.org/10.15488/1704
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1729
dc.contributor.author Harting, Tatjana P.
dc.contributor.author Stubbendorff, Mandy
dc.contributor.author Hammer, Susanne C.
dc.contributor.author Schadzek, Patrik
dc.contributor.author Ngezahayo, Anaclet
dc.contributor.author Escobar, Hugo Murua
dc.contributor.author Nolte, Ingo
dc.date.accessioned 2017-07-17T07:53:16Z
dc.date.available 2017-07-17T07:53:16Z
dc.date.issued 2017
dc.identifier.citation Harting, T.P.; Stubbendorff, M.; Hammer, S.C.; Schadzek, P.; Ngezahayo, A. et al.: Dichloroacetate affects proliferation but not apoptosis in canine mammary cell lines. In: PLoS ONE 12 (2017), Nr. 6, E0178744. DOI: https://doi.org/10.1371/journal.pone.0178744
dc.description.abstract Targeting mitochondrial energy metabolism is a novel approach in cancer research and can be traced back to the description of the Warburg effect. Dichloroacetate, a controversially discussed subject of many studies in cancer research, is a pyruvate dehydrogenase kinase inhibitor. Dichloroacetate causes metabolic changes in cancerous glycolysis towards oxidative phosphorylation via indirect activation of pyruvate dehydrogenase in mitochondria. Canine mammary cancer is frequently diagnosed but after therapy prognosis still remains poor. In this study, canine mammary carcinoma, adenoma and non-neoplastic mammary gland cell lines were treated using 10 mM Dichloroacetate. The effect on cell number, lactate release and PDH expression and cell respiration was investigated. Further, the effect on apoptosis and several apoptotic proteins, proliferation, and microRNA expression was evaluated. Dichloroacetate was found to reduce cell proliferation without inducing apoptosis in all examined cell lines. © 2017 Harting et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. eng
dc.language.iso eng
dc.publisher San Francisco : Public Library of Science
dc.relation.ispartofseries PLoS ONE 12 (2017), Nr. 6
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Canine mammary cancer eng
dc.subject apoptosis eng
dc.subject mitochondria eng
dc.subject carcinomas eng
dc.subject cancer treatment eng
dc.subject pyruvate eng
dc.subject prostate gland eng
dc.subject phosphorylation eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.title Dichloroacetate affects proliferation but not apoptosis in canine mammary cell lines eng
dc.type article
dc.type Text
dc.relation.issn 1932-6203
dc.relation.doi https://doi.org/10.1371/journal.pone.0178744
dc.bibliographicCitation.issue 6
dc.bibliographicCitation.volume 12
dc.bibliographicCitation.firstPage e0178744
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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