dc.identifier.uri |
http://dx.doi.org/10.15488/16755 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/16882 |
|
dc.contributor.author |
Almeria, Ciarra
|
|
dc.contributor.author |
Weiss, René
|
|
dc.contributor.author |
Keck, Maike
|
|
dc.contributor.author |
Weber, Viktoria
|
|
dc.contributor.author |
Kasper, Cornelia
|
|
dc.contributor.author |
Egger, Dominik
|
|
dc.date.accessioned |
2024-03-22T09:47:36Z |
|
dc.date.available |
2024-03-22T09:47:36Z |
|
dc.date.issued |
2024 |
|
dc.identifier.citation |
Almeria, C.; Weiss, R.; Keck, M.; Weber, V.; Kasper, C. et al.: Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system. In: Biotechnology Letters 46 (2024), Nr. 2, S. 279-293. DOI: https://doi.org/10.1007/s10529-024-03465-4 |
|
dc.description.abstract |
Purpose: 3D cell culture and hypoxia have been demonstrated to increase the therapeutic effects of mesenchymal stem/stromal cells (MSCs)-derived extracellular vesicles (EVs). In this study, a process for the production of MSC-EVs in a novel 3D bioreactor system under normoxic and hypoxic conditions was established and the resulting EVs were characterized. Methods: Human adipose-derived MSCs were seeded and cultured on a 3D membrane in the VITVO® bioreactor system for 7 days. Afterwards, MSC-EVs were isolated and characterized via fluorescence nanoparticle tracking analysis, flow cytometry with staining against annexin V (Anx5) as a marker for EVs exposing phosphatidylserine, as well as CD73 and CD90 as MSC surface markers. Results: Cultivation of MSC in the VITVO® bioreactor system demonstrated a higher concentration of MSC-EVs from the 3D bioreactor (9.1 × 109 ± 1.5 × 109 and 9.7 × 109 ± 3.1 × 109 particles/mL) compared to static 2D culture (4.2 × 109 ± 7.5 × 108 and 3.9 × 109 ± 3.0 × 108 particles/mL) under normoxic and hypoxic conditions, respectively. Also, the particle-to-protein ratio as a measure for the purity of EVs increased from 3.3 × 107 ± 1.1 × 107 particles/µg protein in 2D to 1.6 × 108 ± 8.3 × 106 particles/µg protein in 3D. Total MSC-EVs as well as CD73−CD90+ MSC-EVs were elevated in 2D normoxic conditions. The EV concentration and size did not differ significantly between normoxic and hypoxic conditions. Conclusion: The production of MSC-EVs in a 3D bioreactor system under hypoxic conditions resulted in increased EV concentration and purity. This system could be especially useful in screening culture conditions for the production of 3D-derived MSC-EVs. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Dordrecht [u.a.] : Springer Science + Business Media B.V |
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dc.relation.ispartofseries |
Biotechnology Letters 46 (2024), Nr. 2 |
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dc.rights |
CC BY 4.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/4.0 |
|
dc.subject |
3D cell culture |
eng |
dc.subject |
Bioreactors |
eng |
dc.subject |
Extracellular vesicles |
eng |
dc.subject |
Hypoxia |
eng |
dc.subject |
Mesenchymal stem cells |
eng |
dc.subject.ddc |
660 | Technische Chemie
|
|
dc.title |
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1573-6776 |
|
dc.relation.issn |
0141-5492 |
|
dc.relation.doi |
https://doi.org/10.1007/s10529-024-03465-4 |
|
dc.bibliographicCitation.issue |
2 |
|
dc.bibliographicCitation.volume |
46 |
|
dc.bibliographicCitation.firstPage |
279 |
|
dc.bibliographicCitation.lastPage |
293 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|