Scanning laser optical tomography for in toto imaging of the murine cochlea

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dc.identifier.uri Nolte, Lena Tinne, Nadine Schulze, Jennifer Heinemann, Dag Antonopoulos, Georgios C. Meyer, Heiko Nothwang, Hans Gerd Lenarz, Thomas Heisterkamp, Alexander Warnecke, Athanasia Ripken, Tammo 2017-06-21T13:23:20Z 2017-06-21T13:23:20Z 2017
dc.identifier.citation Nolte, L.; Tinne, N.; Schulze, J.; Heinemann, D.; Antonopoulos, G.C. et al.: Scanning laser optical tomography for in toto imaging of the murine cochlea. In: PLoS ONE 12 (2017), Nr. 4, e0175431. DOI:
dc.description.abstract The mammalian cochlea is a complex macroscopic structure due to its helical shape and the microscopic arrangements of the individual layers of cells. To improve the outcomes of hearing restoration in deaf patients, it is important to understand the anatomic structure and composition of the cochlea ex vivo. Hitherto, only one histological technique based on confocal laser scanning microscopy and optical clearing has been developed for in toto optical imaging of the murine cochlea. However, with a growing size of the specimen, e.g., human cochlea, this technique reaches its limitations. Here, we demonstrate scanning laser optical tomography (SLOT) as a valuable imaging technique to visualize the murine cochlea in toto without any physical slicing. This technique can also be applied in larger specimens up to cm3 such as the human cochlea. Furthermore, immunolabeling allows visualization of inner hair cells (otoferlin) or spiral ganglion cells (neurofilament) within the whole cochlea. After image reconstruction, the 3D dataset was used for digital segmentation of the labeled region. As a result, quantitative analysis of position, length and curvature of the labeled region was possible. This is of high interest in order to understand the interaction of cochlear implants (CI) and cells in more detail. © 2017 Nolte et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. eng
dc.description.sponsorship DFG/EXC/1077/1
dc.description.sponsorship Ministry of Lower Saxony
dc.description.sponsorship VolkswagenStiftung
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science
dc.relation.ispartofseries PLoS ONE 12 (2017), Nr. 4
dc.rights CC BY 4.0 Unported
dc.subject Laser eng
dc.subject mammalian cochlea eng
dc.subject cells eng
dc.subject murine cochlea eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Scanning laser optical tomography for in toto imaging of the murine cochlea eng
dc.type article
dc.type Text
dc.relation.issn 1932-6203
dc.bibliographicCitation.issue 4
dc.bibliographicCitation.volume 12
dc.bibliographicCitation.firstPage e0175431
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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