dc.identifier.uri |
http://dx.doi.org/10.15488/16638 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/16765 |
|
dc.contributor.author |
Richter, Alena
|
|
dc.contributor.author |
Li, Yaya
|
|
dc.contributor.author |
Rehbock, Christoph
|
|
dc.contributor.author |
Barcikowski, Stephan
|
|
dc.contributor.author |
Haverich, Axel
|
|
dc.contributor.author |
Wilhelmi, Mathias
|
|
dc.contributor.author |
Böer, Ulrike
|
|
dc.date.accessioned |
2024-03-18T07:44:59Z |
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dc.date.available |
2024-03-18T07:44:59Z |
|
dc.date.issued |
2021 |
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dc.identifier.citation |
Richter, A.; Li, Y.; Rehbock, C.; Barcikowski, S.; Haverich, A. et al.: Triple Modification of Alginate Hydrogels by Fibrin Blending, Iron Nanoparticle Embedding, and Serum Protein-Coating Synergistically Promotes Strong Endothelialization. In: Advanced Materials Interfaces 8 (2021), Nr. 10, 2002015. DOI: https://doi.org/10.1002/admi.202002015 |
|
dc.description.abstract |
Stent therapy can reduce both morbidity and mortality of chronic coronary stenosis and acute myocardial infarction. However, delayed re-endothelialization, endothelial dysfunction, and chronic inflammation are still unsolved problems. Alginate hydrogels can be used as a coating for stent surfaces; however, complete and fast endothelialization cannot be achieved. In this study, alginate hydrogels are modified by fibrin blending, iron nanoparticle (Fe-NP) embedding, and serum protein coating (SPC) while surface properties and endothelialization capacity are monitored. Only a triple, synergetic modification of the alginate coating by simultaneous I) fibrin blending, II) Fe-NP addition complemented by III) SPC is found to significantly improve endothelial cell viability (live–dead-staining) and proliferation (WST-8 assay). These conditions yield formation of closed endothelial cell monolayers and an up to threefold increase (p < 0.01) in viability, while, interestingly, no effect is found when the modifications (I)–(III) are conducted individually. This synergetic effect is attributed to an accumulation of agglomerated Fe-NP and serum proteins along fibrin fibers, observed via laser scanning microscopy tracking nanoparticle scattering and tetramethylrhodamine (TRITC)-albumin fluorescence. These synergetic effects can pave the way toward a novel strategy for the modification of various hydrogel-based biomaterials and biomaterial coatings. |
eng |
dc.language.iso |
eng |
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dc.publisher |
Weinheim : Wiley-VCH |
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dc.relation.ispartofseries |
Advanced Materials Interfaces 8 (2021), Nr. 10 |
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dc.rights |
CC BY-NC-ND 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
|
dc.subject |
alginate-fibrin hydrogels |
eng |
dc.subject |
endothelialization |
eng |
dc.subject |
iron nanoparticles |
eng |
dc.subject |
laser ablation in liquids |
eng |
dc.subject |
serum protein adsorption |
eng |
dc.subject.ddc |
540 | Chemie
|
|
dc.subject.ddc |
600 | Technik
|
|
dc.title |
Triple Modification of Alginate Hydrogels by Fibrin Blending, Iron Nanoparticle Embedding, and Serum Protein-Coating Synergistically Promotes Strong Endothelialization |
eng |
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.essn |
2196-7350 |
|
dc.relation.issn |
2196-7350 |
|
dc.relation.doi |
https://doi.org/10.1002/admi.202002015 |
|
dc.bibliographicCitation.issue |
10 |
|
dc.bibliographicCitation.volume |
8 |
|
dc.bibliographicCitation.firstPage |
2002015 |
|
dc.description.version |
publishedVersion |
eng |
tib.accessRights |
frei zug�nglich |
|
dc.bibliographicCitation.articleNumber |
2002015 |
|