dc.identifier.uri |
http://dx.doi.org/10.15488/16399 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/16526 |
|
dc.contributor.author |
Heuer, Christopher
|
|
dc.contributor.author |
Leonard, Heidi
|
|
dc.contributor.author |
Nitzan, Nadav
|
|
dc.contributor.author |
Lavy-Alperovitch, Ariella
|
|
dc.contributor.author |
Massad-Ivanir, Naama
|
|
dc.contributor.author |
Scheper, Thomas
|
|
dc.contributor.author |
Segal, Ester
|
|
dc.date.accessioned |
2024-02-27T07:23:26Z |
|
dc.date.available |
2024-02-27T07:23:26Z |
|
dc.date.issued |
2020 |
|
dc.identifier.citation |
Heuer, C.; Leonard, H.; Nitzan, N.; Lavy-Alperovitch, A.; Massad-Ivanir, N. et al.: Antifungal Susceptibility Testing of Aspergillus Niger on Silicon Microwells by Intensity-Based Reflectometric Interference Spectroscopy. In: ACS Infectious Diseases 6 (2020), Nr. 10, S. 2560-2566. DOI: https://doi.org/10.1021/acsinfecdis.0c00234 |
|
dc.description.abstract |
There is a demonstrated and paramount need for rapid, reliable infectious disease diagnostics, particularly those for invasive fungal infections. Current clinical determinations for an appropriate antifungal therapy can take up to 3 days using current antifungal susceptibility testing methods, a time-to-readout that can prove detrimental for immunocompromised patients and promote the spread of antifungal resistant pathogens. Herein, we demonstrate the application of intensity-based reflectometric interference spectroscopic measurements (termed iPRISM) on microstructured silicon sensors for use as a rapid, phenotypic antifungal susceptibility test. This diagnostic platform optically tracks morphological changes of fungi corresponding to conidia growth and hyphal colonization at a solid-liquid interface in real time. Using Aspergillus niger as a model fungal pathogen, we can determine the minimal inhibitory concentration of clinically relevant antifungals within 12 h. This assay allows for expedited detection of fungal growth and provides a label-free alternative to broth microdilution and agar diffusion methods, with the potential to be used for point-of-care diagnostics. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Washington, DC : ACS Publ. |
|
dc.relation.ispartofseries |
ACS Infectious Diseases 6 (2020), Nr. 10 |
|
dc.rights |
ACS AuthorChoice |
|
dc.rights.uri |
https://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html |
|
dc.subject |
antifungal susceptibility testing |
eng |
dc.subject |
Aspergillus Niger |
eng |
dc.subject |
fungal resistance |
eng |
dc.subject |
optical sensor |
eng |
dc.subject |
sensor |
eng |
dc.subject.ddc |
540 | Chemie
|
|
dc.subject.ddc |
610 | Medizin, Gesundheit
|
|
dc.title |
Antifungal Susceptibility Testing of Aspergillus Niger on Silicon Microwells by Intensity-Based Reflectometric Interference Spectroscopy |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
2373-8227 |
|
dc.relation.doi |
https://doi.org/10.1021/acsinfecdis.0c00234 |
|
dc.bibliographicCitation.issue |
10 |
|
dc.bibliographicCitation.volume |
6 |
|
dc.bibliographicCitation.firstPage |
2560 |
|
dc.bibliographicCitation.lastPage |
2566 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|