The standard treatment of human visceral leishmaniasis involves the use of pentavalent antimony (Sbv). Its mechanism of action is unknown because of the limited information available about intracellular antimony metabolism and about the genes that regulate these processes. Herein, flow injection-inductively coupled plasma mass spectrometry (ICP-MS), flow injection hydride generation ICP-MS, and ion chromatography ICP-MS were used to measure antimony accumulation and intracellular metabolism in the human protozoan parasite Leishmania donovani. Amastigotes (the intracellular form) and promastigotes (the extracellular form) accumulate Sbv and Sb III via separate transport systems. Stage-specific intracellular Sbv reducing activity was apparent in amastigotes, which reduced the negligibly toxic Sbv to highly toxic SbIII. This amastigote-specific reducing activity was deficient in the Pentostam-resistant mutant L. donovani Ld1S.20. These data indicate that parasite susceptibility to Sbv correlates with its level of Sbv reducing activity. Also, in promastigotes of both wild-type L. donovani and the Pentostam-resistant mutant L. donovani Ld1S.20, Sbv inhibited the toxicity of SbIII but not of AsIII. Both Sbv and SbIII were toxic to wild-type amastigotes. However, as observed in promastigotes, in mutant amastigotes Sbv inhibits Sb III but not AsIII activity. Anion exchange chromatography showed that intracellular antimony metabolism occurred in both promastigotes and amastigotes. These data demonstrate that the interaction between the two antimony oxidation states occurs intracellularly, within the parasite. The results also indicate that Sbv anti-leishmanial activity is dependent on its reduction to SbIII. The mechanism of this novel intracellular Sbv reduction has yet to be identified, and it may or may not be enzymatic. This is the first description of intracellular Sb v reducing activity in Leishmania as well as in any prokaryotic or eukaryotic cell.
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