Occurrence of large-scale mitochondrial DNA deletions in human colorectal cancer

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dc.identifier.uri http://dx.doi.org/10.15488/1500
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1525
dc.contributor.author Akouchekian, Mansoureh
dc.contributor.author Houshmand, Massoud
dc.contributor.author Hemati, Simin
dc.contributor.author Shafa, Mehdi
dc.date.accessioned 2017-05-10T13:25:06Z
dc.date.available 2017-05-10T13:25:06Z
dc.date.issued 2008
dc.identifier.citation Akouchekian, M.; Houshmand, M.; Hemati, S.; Shafa, M.: Occurrence of large-scale mitochondrial DNA deletions in human colorectal cancer. In: Archives of Medical Science 4 (2008)Nr. 3, S. 249-253.
dc.description.abstract Introduction: The aim of this study was to determine the mutation patterns of colon cancers through screening of different regions of mitochondrial DNA (mtDNA) in colon cancer patients. Material and methods: In order to investigate whether deletions exist in the mitochondrial DNA of colon cancer patients, we used a PCR assay to assess the presence of large-scale deletions. We screened four regions of the mitochondrial genome by PCR amplification and Southern blot analysis followed by DNA sequencing. Previously, deficiency in mitochondrial complex I has been reported; therefore we focused on the region of mtDNA that encodes the genes of this complex. Results: In 11 out of 90 patients, we found an 8.7 kb deletion. Large-scale deletions of mtDNA are common events that have been found to occur in human ageing and in patients with mitochondrial myopathies. Based on our results the mtDNA 8.7 kb deletion occurs in 12.2% of the colorectal cancer (CRC) samples. Conclusions: As reactive oxygen species (ROS) are continuously generated by the respiratory chain, they may cause significant oxidative damage to mtDNA (for example mtDNA deletions or mutations) if not efficiently eliminated. Defective respiratory enzymes containing protein subunits encoded by the deleted mtDNA may further enhance free radical production, resulting in more profound oxidative damage in CRC patients. Copyright © 2008 Termedia & Banach. eng
dc.language.iso eng
dc.publisher Poznan : Termedia Publishing House
dc.relation.ispartofseries Archives of Medical Science 4 (2008)Nr. 3
dc.rights CC BY-NC-SA 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject 8.7 kb deletion eng
dc.subject Colon cancer eng
dc.subject Large-scale deletion eng
dc.subject Mitochondrial DNA eng
dc.subject mitochondrial DNA eng
dc.subject adult eng
dc.subject aged eng
dc.subject aging eng
dc.subject article eng
dc.subject colorectal cancer eng
dc.subject controlled study eng
dc.subject DNA flanking region eng
dc.subject DNA sequence eng
dc.subject female eng
dc.subject gene deletion eng
dc.subject genetic screening eng
dc.subject human eng
dc.subject major clinical study eng
dc.subject male eng
dc.subject mitochondrial DNA disorder eng
dc.subject mitochondrial myopathy eng
dc.subject multiplex polymerase chain reaction eng
dc.subject mutational analysis eng
dc.subject oxidative stress eng
dc.subject sequence analysis eng
dc.subject Southern blotting eng
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Occurrence of large-scale mitochondrial DNA deletions in human colorectal cancer
dc.type Article
dc.type Text
dc.relation.essn 1896-9151
dc.relation.issn 1734-1922
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 4
dc.bibliographicCitation.firstPage 249
dc.bibliographicCitation.lastPage 253
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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