One pathway, many compounds: heterologous expression of a fungal biosynthetic pathway reveals its intrinsic potential for diversity

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dc.identifier.uri http://dx.doi.org/10.15488/125
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/143
dc.contributor.author Wasil, Zahida
dc.contributor.author Pahirulzaman, Khomaizon A.K.
dc.contributor.author Butts, Craig
dc.contributor.author Simpson, Thomas J.
dc.contributor.author Lazarus, Colin M.
dc.contributor.author Cox, Russell J.
dc.date.accessioned 2015-12-03T10:13:27Z
dc.date.available 2015-12-03T10:13:27Z
dc.date.issued 2013-08-08
dc.identifier.citation Wasil, Zahida; Pahirulzaman, Khomaizon A. K.; Butts, Craig; Simpson, Thomas J.; Lazarus, Colin M.; Cox, Russell J.: One pathway, many compounds: heterologous expression of a fungal biosynthetic pathway reveals its intrinsic potential for diversity. In: Chemical Science 4 (2013), Nr. 1, S. 3845-3856. DOI: http://dx.doi.org/10.1039/c3sc51785c
dc.description.abstract Heterologous expression of genes from the proposed aspyridone biosynthetic cluster from Aspergillus nidulans in the host Aspergillus oryzae led to the production of eight different compounds in addition to aspyridone A 1, one of the previously observed products. The pathway genes were incapable of producing aspyridone B 2, the previously accepted final product of the pathway. Expression of restricted sets of genes in addition to the core polyketide synthase – nonribosomal peptide synthetase (PKS-NRPS) genes apdA and apdC revealed: that apdE encodes a cytochrome P450 enzyme with ring-expanding and unprecedented dephenylation activity; that apdB encodes an N-hydroxylase, an activity not previously suspected; that the productivity of ApdA and ApdC proteins appears to be significantly enhanced in the presence of the downstream ApdE oxidase; and no obvious chemical roles for ApdD and ApdG. Furthermore, the ApdC enoyl reductase appears to operate with different stereoselectivity in different PKS cycles. All of these features illustrate the inherent diversity of compounds potentially produced by the apd pathway and the high utility of a whole pathway expression strategy for investigating and revealing new biosynthetic chemistry in fungi. eng
dc.description.sponsorship Government of Pakistan
dc.description.sponsorship Majlis Amanah Rakyat (MARA) Malaysia
dc.description.sponsorship EP/F066104/1
dc.language.iso eng eng
dc.publisher Cambridge : Royal Society of Chemistry
dc.relation.ispartofseries Chemical Science 4 (2013), Nr. 10
dc.rights CC BY 3.0
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject polyketide synthase eng
dc.subject aspergillus-nidulans eng
dc.subject chemical diversity eng
dc.subject filamentous fungi eng
dc.subject gene clusters eng
dc.subject proteins eng
dc.subject tenellin eng
dc.subject.ddc 540 | Chemie ger
dc.title One pathway, many compounds: heterologous expression of a fungal biosynthetic pathway reveals its intrinsic potential for diversity eng
dc.type article
dc.relation.issn 2041-6520
dc.relation.doi http://dx.doi.org/10.1039/c3sc51785c
dc.bibliographicCitation.issue 10
dc.bibliographicCitation.volume 4
dc.bibliographicCitation.firstPage 3845
dc.bibliographicCitation.lastPage 3856
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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