Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

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dc.identifier.uri http://dx.doi.org/10.15488/122
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/140
dc.contributor.author Song, Zhongshu
dc.contributor.author Bakeer, Walid
dc.contributor.author Marshall, James W.
dc.contributor.author Yakasai, Ahmed A.
dc.contributor.author Khalid, Rozida Mohd
dc.contributor.author Collemare, Jerome
dc.contributor.author Skellam, Elizabeth
dc.contributor.author Tharreau, Didier
dc.contributor.author Lebrun, Marc-Henri
dc.contributor.author Lazarus, Colin M.
dc.contributor.author Bailey, Andrew M.
dc.contributor.author Simpson, Thomas J.
dc.contributor.author Cox, Russell J.
dc.date.accessioned 2015-12-03T10:13:26Z
dc.date.available 2015-12-03T10:13:26Z
dc.date.issued 2015-06-01
dc.identifier.citation Song, Zhongshu; Bakeer, Walid; Marshall, James W.; Yakasai, Ahmed A.; Khalid, Rozida Mohd; Collemare, Jerome; Skellam, Elizabeth; Tharreau, Didier; Lebrun, Marc-Henri; Lazarus, Colin M.; Bailey, Andrew M.; Simpson, Thomas J.; Cox, Russell J.: Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. In: Chemical Science 6 (2015), Nr. 8, S. 4837-4845. DOI: http://dx.doi.org/10.1039/c4sc03707c
dc.description.abstract The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound. eng
dc.description.sponsorship Government of Egypt Scholarship
dc.description.sponsorship The School of Chemistry at the University of Bristol and the Mark Evans Scholarship
dc.description.sponsorship Kano State Government Nigeria
dc.description.sponsorship MacArthur Foundation
dc.description.sponsorship Bayero University
dc.description.sponsorship Nigerian Petroleum Technology Fund
dc.description.sponsorship Malaysian Govenment Scholarship
dc.description.sponsorship EP/F066104/1
dc.language.iso eng eng
dc.publisher Cambridge : Royal Society of Chemistry
dc.relation.ispartofseries Chemical Science 6 (2015), Nr. 8
dc.rights CC BY 3.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject synthase-peptide synthetase eng
dc.subject biosynthesis eng
dc.subject cluster eng
dc.subject grisea eng
dc.subject identification eng
dc.subject proteins eng
dc.subject cytochalasin eng
dc.subject diaporthichalasin eng
dc.subject phomopsichalasin eng
dc.subject chaetoglobosin eng
dc.subject.ddc 540 | Chemie ger
dc.title Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae eng
dc.type Article
dc.type Text
dc.relation.essn 2041-6539
dc.relation.issn 2041-6520
dc.relation.doi http://dx.doi.org/10.1039/c4sc03707c
dc.bibliographicCitation.issue 8
dc.bibliographicCitation.volume 6
dc.bibliographicCitation.firstPage 4837
dc.bibliographicCitation.lastPage 4845
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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