Fluorescence Masking Based Multifunctional Quantum Dots’ Assay for HSP90α Interactions Detection

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dc.identifier.uri http://dx.doi.org/10.15488/13356
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/13465
dc.contributor.author Kishore, Anusha
dc.contributor.author Fan, Lu
dc.contributor.author Stahl, Frank
dc.contributor.author Reichel, Thomas
dc.contributor.author Krüger, Karsten
dc.contributor.author Zeilinger, Carsten
dc.date.accessioned 2023-03-21T06:08:06Z
dc.date.available 2023-03-21T06:08:06Z
dc.date.issued 2023
dc.identifier.citation Kishore, A.; Fan, L.; Stahl, F.; Reichel, T.; Krüger, K. et al.: Fluorescence Masking Based Multifunctional Quantum Dots’ Assay for HSP90α Interactions Detection. In: Applied Sciences : open access journal 13 (2023), Nr. 5, 2957. DOI: https://doi.org/10.3390/app13052957
dc.description.abstract HSP90α is one of the most common stress proteins in cells; hence, it is a good target for developing drugs and testing systems for cancer or physical stress levels in humans. Streptavidin conjugated quantum dots (Sav-QDs) are widely used as fluorophores for biosensing to overcome chemical labelling problems. In this work, we have attempted to develop a multifunctional and robust assay for HSP90α. The detection technique was based on the masking of the fluorescence of spotted Sav-QDs on nitrocellulose chips (NC). Biotinylated ligand/antibody attaches to the spotted Sav-QD and then HSP90α is attached, which causes the masking of fluorescence. The masking of fluorescence was used to detect protein–ligand interactions, the effect of inhibitors, protein–protein interactions, and the presence of protein in the biological sample. The load of detection (LoD) of the assay lies in the nano molar range, making it a sensitive assay. The results from the experiments suggest that the used approach is promising for developing a multifunctional, robust, and sensitive assay for proteins that can be used for point-of-care detection in complex biological samples. eng
dc.language.iso eng
dc.publisher Basel : MDPI
dc.relation.ispartofseries Applied Sciences : open access journal 13 (2023), Nr. 5
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject quantum dots eng
dc.subject Sav-QD eng
dc.subject biotin-ATP eng
dc.subject biotin-HSP90 antibody eng
dc.subject HSP90α eng
dc.subject fluorescence masking eng
dc.subject.ddc 600 | Technik ger
dc.title Fluorescence Masking Based Multifunctional Quantum Dots’ Assay for HSP90α Interactions Detection
dc.type Article
dc.type Text
dc.relation.essn 2076-3417
dc.relation.doi https://doi.org/10.3390/app13052957
dc.bibliographicCitation.issue 5
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage 2957
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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