Monitoring of microalgal cultivations with on-line, flow-through microscopy

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dc.identifier.uri Havlik, Ivo Reardon, Kenneth F. Ünal, Mehmet Lindner, Patrick Prediger, Andreas Babitzky, Alexander Beutel, Sascha Scheper, Thomas 2017-04-20T08:42:16Z 2017-04-20T08:42:16Z 2013
dc.identifier.citation Havlik, I.; Reardon, K.F.; Ünal, M.; Lindner, P.; Prediger, A. et al.: Monitoring of microalgal cultivations with on-line, flow-through microscopy. In: Algal Research 2 (2013), Nr. 3, S. 253-257. DOI:
dc.description.abstract Microalgal cultivations present challenges for monitoring and process control posed by their large scale and the likelihood that they will be composed of multiple species. Cell concentration is a fundamental parameter in any cultivation but is typically measured using off-line methods that may be time-consuming, laborious, or subject to interferences. Here, an in-situ microscope has been adapted for monitoring microalgal cultivations by adding a flow-through cell and adjusting image-processing algorithms. After installation in the bypass of a photobioreactor, the microscope enabled the continuous, automated acquisition of cell count, cell size, and cell morphology data on-line during cultivation processes over a period of 20. days, without sampling. The flow-through microscope was tested in cultivations of Chlamydomonas reinhardtii and Chlorella vulgaris. Cell concentration measurements were in agreement with off-line optical density measurements for both species. In addition, cell size and morphology distributions were obtained that revealed population shifts during the cultivation of C. vulgaris. This monitoring system thus provides a means to obtain detailed, non-invasive insights of microalgal cultivation processes. eng
dc.description.sponsorship Jud and Pat Harper Professorship in Chemical and Biological Engineering
dc.description.sponsorship Sustainable Bioenergy Development Center of Colorado State University
dc.language.iso eng
dc.publisher Amsterdam : Elsevier
dc.relation.ispartofseries Algal Research 2 (2013), Nr. 3
dc.rights CC BY-NC-ND 4.0 Unported
dc.subject Automated image processing eng
dc.subject Cell count eng
dc.subject Cell size distribution eng
dc.subject Flow-through microscopy eng
dc.subject Microalgae eng
dc.subject Chlamydomonas reinhardtii eng
dc.subject Chlorella vulgaris eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 580 | Pflanzen (Botanik) ger
dc.title Monitoring of microalgal cultivations with on-line, flow-through microscopy
dc.type article
dc.type Text
dc.relation.essn 2211-9264
dc.relation.doi 10.1016/j.algal.2013.04.001
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 2
dc.bibliographicCitation.firstPage 253
dc.bibliographicCitation.lastPage 257
dc.description.version acceptedVersion
tib.accessRights frei zug�nglich

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