dc.identifier.uri |
http://dx.doi.org/10.15488/13132 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/13237 |
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dc.contributor.author |
Rahim, Muhammad Abdul
|
|
dc.contributor.author |
Yasmin, Adeela
|
|
dc.contributor.author |
Imran, Muhammad
|
|
dc.contributor.author |
Nisa, Mahr Un
|
|
dc.contributor.author |
Khalid, Waseem
|
|
dc.contributor.author |
Esatbeyoglu, Tuba
|
|
dc.contributor.author |
Korma, Sameh A.
|
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dc.date.accessioned |
2022-12-12T14:52:29Z |
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dc.date.available |
2022-12-12T14:52:29Z |
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dc.date.issued |
2022 |
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dc.identifier.citation |
Rahim, M.A.; Yasmin, A.; Imran, M.; Nisa, M.U.; Khalid, W. et al.: Optimization of the Ultrasound Operating Conditions for Extraction and Quantification of Fructooligosaccharides from Garlic (Allium sativum L.) via High-Performance Liquid Chromatography with Refractive Index Detector. In: Molecules : a journal of synthetic chemistry and natural product chemistry 27 (2022), Nr. 19, 6388. DOI: https://doi.org/10.3390/molecules27196388 |
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dc.description.abstract |
Dietary interventions have captured the attention of nutritionists due to their health-promoting aspects, in addition to medications. In this connection, supplementation of nutraceuticals is considered as a rational approach to alleviating various metabolic disorders. Among novel strategies, prebiotic-supplemented foods are an encouraging trend in addressing the issue. In the present investigation, prebiotic fructooligosaccharides (FOS) were extracted from garlic (Allium sativum L.) powder using ultrasound-assisted extraction (UAE). The response surface methodology (RSM) was used to optimize the independent sonication variables, i.e., extraction temperature (ET, 80, 90, and 100 °C), amplitude level (AL, 70, 80, and 90%) and sonication time (ST, 10, 15 and 20 min). The maximum FOS yield (6.23 ± 0.52%) was obtained at sonication conditions of ET (80 °C), AL (80%) and ST (10 min), while the minimum yield of FOS was obtained at high operating temperatures and time. The optimized FOS yield (7.19%) was obtained at ET (80 °C), AL (73%) and ST (15 min) after model validation. The influence of sonication parameters, i.e., ET, AL and ST, on FOS yield was evaluated by varying their coded levels from −1 to +1, respectively, for each independent variable. High-performance liquid chromatography with refractive index detector (HPLC-RID) detection and quantification indicated that sucrose was present in high amounts (2.06 ± 0.10 g/100 g) followed by fructose and glucose. Total FOS fractions which included nystose present in maximum concentration (526 ± 14.7 mg/100 g), followed by 1-kestose (428 ± 19.5 mg/100 g) and fructosylnystoses (195 ± 6.89 mg/100 g). Conclusively, garlic is a good source of potential prebiotics FOS and they can be extracted using optimized sonication parameters using ultrasound-assisted techniques with maximum yield percentage. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Basel : MDPI |
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dc.relation.ispartofseries |
Molecules : a journal of synthetic chemistry and natural product chemistry 27 (2022), Nr. 19 |
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dc.rights |
CC BY 4.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
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dc.subject |
garlic (Allium sativum L.) |
eng |
dc.subject |
FOS |
eng |
dc.subject |
prebiotics |
eng |
dc.subject |
UAE |
eng |
dc.subject |
RSM |
eng |
dc.subject |
HPLC-RID |
eng |
dc.subject |
spice |
eng |
dc.subject |
detection quantification |
eng |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Optimization of the Ultrasound Operating Conditions for Extraction and Quantification of Fructooligosaccharides from Garlic (Allium sativum L.) via High-Performance Liquid Chromatography with Refractive Index Detector |
eng |
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.essn |
1420-3049 |
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dc.relation.doi |
https://doi.org/10.3390/molecules27196388 |
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dc.bibliographicCitation.issue |
19 |
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dc.bibliographicCitation.volume |
27 |
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dc.bibliographicCitation.firstPage |
6388 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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