Polysialic acid production using Escherichia coli K1 in a disposable bag reactor

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dc.identifier.uri http://dx.doi.org/10.15488/1261
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1286
dc.contributor.author de Vries, Ingo
dc.contributor.author Busse, Christoph
dc.contributor.author Kopatz, Jens
dc.contributor.author Neumann, Harald
dc.contributor.author Beutel, Sascha
dc.contributor.author Scheper, Thomas
dc.date.accessioned 2017-04-05T12:01:24Z
dc.date.available 2018-02-13T23:05:13Z
dc.date.issued 2017
dc.identifier.citation de Vries, I.; Busse, C.; Kopatz, J.; Neumann, H.; Beutel, S.; Scheper, T.: Polysialic acid production using Escherichia coli K1 in a disposable bag reactor. In: Engineering in Life Sciences 17 (2017), Nr. 7, S. 723–731. DOI: https://doi.org/10.1002/elsc.201600220
dc.description.abstract Polysialic acid (polySia), consisting of α-(2,8)-linked N-acetylneuraminic acid monomers plays a crucial role in many biological processes. This study presents a novel process for the production of endogenous polySia using Escherichia coli K1 in a disposable bag reactor with wave-induced mixing. Disposable bag reactors provide easy and fast production in terms of regulatory requirements as GMP, flexibility, and can easily be adjusted to larger production capacities not only by scale up but also by parallelization. Due to the poor oxygen transfer rate compared to a stirred tank reactor, pure oxygen was added during the cultivation to avoid oxygen limitation. During the exponential growth phase the growth rate was 0.61 h−1. Investigation of stress-related product release from the cell surface showed no significant differences between the disposable bag reactor with wave-induced mixing and the stirred tank reactor. After batch cultivation a cell dry weight of 6.8 g L−1 and a polySia concentration of 245 mg L−1 were reached. The total protein concentration in the supernatant was 132 mg L−1. After efficient and time-saving downstream processing characterization of the final product showed a protein content of below 0.04 mgprotein/gpolySia and a maximal chain length of ∼90 degree of polymerization. This is the peer reviewed version of the following article: de Vries, I.; Busse, C.; Kopatz, J.; Neumann, H.; Beutel, S.; Scheper, T.: Polysialic acid production using Escherichia coli K1 in a disposable bag reactor. In: Engineering in Life Sciences (2017), which has been published in final form at https://doi.org/10.1002/elsc.201600220. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. eng
dc.description.sponsorship DFG/NE507/14-1
dc.description.sponsorship DFG/SCHE279/35-1
dc.description.sponsorship BMBF/03VP00271
dc.description.sponsorship BMBF/03VP00273
dc.language.iso eng
dc.publisher Weinheim : Wiley-VCH Verlag
dc.relation.ispartofseries Engineering in Life Sciences 17 (2017), Nr. 7
dc.rights Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden.
dc.subject Disposable bag reactor eng
dc.subject Escherichia coli K1 eng
dc.subject Oxygen limitation eng
dc.subject Polysialic acid eng
dc.subject Stirred tank reactor eng
dc.subject Cell membranes eng
dc.subject Mixing eng
dc.subject Oxygen eng
dc.subject Proteins eng
dc.subject Tanks (containers) eng
dc.subject Degree of polymerization eng
dc.subject Disposable bag reactor eng
dc.subject Exponential growth phase eng
dc.subject Oxygen limitation eng
dc.subject Polysialic acid eng
dc.subject Regulatory requirements eng
dc.subject Stirred tank reactors eng
dc.subject Total protein concentration eng
dc.subject Escherichia coli eng
dc.subject.ddc 620 | Ingenieurwissenschaften und Maschinenbau ger
dc.title Polysialic acid production using Escherichia coli K1 in a disposable bag reactor
dc.type Text
dc.type article
dc.relation.issn 1618-0240
dc.relation.doi https://doi.org/10.1002/elsc.201600220
dc.bibliographicCitation.issue 7
dc.bibliographicCitation.volume 17
dc.bibliographicCitation.firstPage 723
dc.bibliographicCitation.lastPage 731
dc.description.version acceptedVersion
tib.accessRights frei zug�nglich


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