Loading equine oocytes with cryoprotective agents captured with a finite element method model

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dc.identifier.uri http://dx.doi.org/10.15488/12453
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/12552
dc.contributor.author Içli, Sercan
dc.contributor.author Soleimani, Meisan
dc.contributor.author Oldenhof, Harriëtte
dc.contributor.author Sieme, Harald
dc.contributor.author Wriggers, Peter
dc.contributor.author Wolkers, Willem F.
dc.date.accessioned 2022-07-07T08:09:57Z
dc.date.available 2022-07-07T08:09:57Z
dc.date.issued 2021
dc.identifier.citation Içli, S.; Soleimani, M.; Oldenhof, H.; Sieme, H.; Wriggers, P. et al.: Loading equine oocytes with cryoprotective agents captured with a finite element method model. In: Scientific Reports 11 (2021), Nr. 1, 19812. DOI: https://doi.org/10.1038/s41598-021-99287-9
dc.description.abstract Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect the oocytes against freezing injury. The intracellular introduction of CPAs, however, may cause irreversible osmotic damage. The response of cells exposed to CPA solutions is governed by the permeability of the cellular membrane towards water and the CPAs. In this study, a mathematical mass transport model describing the permeation of water and CPAs across an oocyte membrane was used to simulate oocyte volume responses and concomitant intracellular CPA concentrations during the exposure of oocytes to CPA solutions. The results of the analytical simulations were subsequently used to develop a phenomenological finite element method (FEM) continuum model to capture the response of oocytes exposed to CPA solutions with spatial information. FEM simulations were used to depict spatial differences in CPA concentration during CPA permeation, namely at locations near the membrane surface and towards the middle of the cell, and to capture corresponding changes in deformation and hydrostatic pressure. FEM simulations of the multiple processes occurring during CPA loading of oocytes are a valuable tool to increase our understanding of the mechanisms underlying cryopreservation outcome. © 2021, The Author(s). eng
dc.language.iso eng
dc.publisher London : Nature Publishing Group
dc.relation.ispartofseries Scientific Reports 11 (2021), Nr. 1
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject cryoprotective agent eng
dc.subject animal eng
dc.subject cell membrane permeability eng
dc.subject cryopreservation eng
dc.subject cytology eng
dc.subject horse eng
dc.subject oocyte eng
dc.subject procedures eng
dc.subject vitrification eng
dc.subject Animals eng
dc.subject Cell Membrane Permeability eng
dc.subject Cryopreservation eng
dc.subject Cryoprotective Agents eng
dc.subject Horses eng
dc.subject Oocytes eng
dc.subject Vitrification eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 600 | Technik ger
dc.title Loading equine oocytes with cryoprotective agents captured with a finite element method model
dc.type Article
dc.type Text
dc.relation.essn 2045-2322
dc.relation.doi https://doi.org/10.1038/s41598-021-99287-9
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 11
dc.bibliographicCitation.firstPage 19812
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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