3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation

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dc.identifier.uri http://dx.doi.org/10.15488/12339
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/12438
dc.contributor.author Frey, Lasse Jannis
dc.contributor.author Vorländer, David
dc.contributor.author Ostsieker, Hendrik
dc.contributor.author Rasch, Detlev
dc.contributor.author Lohse, Jan-Luca
dc.contributor.author Breitfeld, Maximilian
dc.contributor.author Grosch, Jan-Hendrik
dc.contributor.author Wehinger, Gregor D.
dc.contributor.author Bahnemann, Janina
dc.contributor.author Krull, Rainer
dc.date.accessioned 2022-06-27T04:36:59Z
dc.date.available 2022-06-27T04:36:59Z
dc.date.issued 2021
dc.identifier.citation Frey, L.J.; Vorländer, D.; Ostsieker, H.; Rasch, D.; Lohse, J.-L. et al.: 3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation. In: Scientific Reports 11 (2021), Nr. 1, 7276. DOI: https://doi.org/10.1038/s41598-021-86654-9
dc.description.abstract With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with kLa values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development. © 2021, The Author(s). eng
dc.language.iso eng
dc.publisher London : Nature Publishing Group
dc.relation.ispartofseries Scientific Reports 11 (2021), Nr. 1
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Saccharomyces-Cerevisiae eng
dc.subject Oxygen-Transfer eng
dc.subject Mass-Transfer eng
dc.subject Growth eng
dc.subject Glucose eng
dc.subject System eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 600 | Technik ger
dc.title 3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation
dc.type article
dc.type Text
dc.relation.essn 2045-2322
dc.relation.doi https://doi.org/10.1038/s41598-021-86654-9
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 11
dc.bibliographicCitation.firstPage 7276
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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