Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy

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dc.identifier.uri http://dx.doi.org/10.15488/1214
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1238
dc.contributor.author Koch, Matthias
dc.contributor.author Zagermann, Serge
dc.contributor.author Kniggendorf, Ann-Kathrin
dc.contributor.author Meinhardt-Wollweber, Merve
dc.contributor.author Roth, Bernhard
dc.date.accessioned 2017-03-20T13:19:20Z
dc.date.available 2018-02-17T23:05:13Z
dc.date.issued 2017
dc.identifier.citation Koch, M.; Zagermann, S.; Kniggendorf, A.-K.; Meinhardt-Wollweber, M.; Roth, B.: Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy. In: Journal of Raman Spectroscopy 48 (2017), Nr. 5, S. 686–691. DOI: https://doi.org/10.1002/jrs.5102
dc.description.abstract Measuring the kinetics of the violaxanthin cycle imposes an experimental challenge. Traditionally, carotenoid analysis was carried out laboriously with high-performance liquid chromatography. In this work, we present the first in vivo approach to directly measure the kinetics of the violaxanthin cycle, using resonance Raman spectroscopy in combination with baseline correction and principal component analysis. Applying the new approach allows measuring thousands of data points as opposed to the few possible with chemical analysis over the course of a violaxanthin cycle kinetics experiment. In vivo analysis of the violaxanthin cycle is necessary to fully understand adaptation kinetics to varying light conditions, the knowledge of which is especially important for assessing the stress tolerance of plants in the wake of the increasing climate change. Three experiments on the green alga Dunaliella salina were performed, featuring both the light-to-dark and dark-to-light transitions response of the algae. This is the peer reviewed version of the following article: Koch, M. et al.: Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy. In: Journal of Raman Spectroscopy (2017), which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.5102/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. eng
dc.language.iso eng
dc.publisher Chichester : John Wiley and Sons Ltd
dc.relation.ispartofseries Journal of Raman Spectroscopy 48 (2017), Nr. 5
dc.rights Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden.
dc.subject Dunaliellasalina eng
dc.subject Kinetics eng
dc.subject Resonance Raman eng
dc.subject Stress adaptation eng
dc.subject Violaxanthin cycle eng
dc.subject.ddc 620 | Ingenieurwissenschaften und Maschinenbau ger
dc.subject.ddc 510 | Mathematik ger
dc.subject.ddc 530 | Physik ger
dc.title Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy
dc.type Text
dc.type article
dc.relation.issn 0377-0486
dc.relation.doi https://doi.org/10.1002/jrs.5102
dc.bibliographicCitation.issue 5
dc.bibliographicCitation.volume 48
dc.bibliographicCitation.firstPage 686
dc.bibliographicCitation.lastPage 691
dc.description.version acceptedVersion
tib.accessRights frei zug�nglich


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