Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases

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dc.identifier.uri http://dx.doi.org/10.15488/1184
dc.identifier.uri http://www.repo.uni-hannover.de/handle/123456789/1208
dc.contributor.author Vorhölter, Frank-Jörg
dc.contributor.author Wiggerich, Heinrich-Günter
dc.contributor.author Scheidle, Heiko
dc.contributor.author Sidhu, Vishaldeep Kaur
dc.contributor.author Mrozek, Kalina
dc.contributor.author Küster, Helge
dc.contributor.author Pühler, Alfred
dc.contributor.author Niehaus, Karsten
dc.date.accessioned 2017-03-02T14:05:10Z
dc.date.available 2017-03-02T14:05:10Z
dc.date.issued 2012
dc.identifier.citation Vorhoelter, Frank-Joerg; Wiggerich, Heinrich-Guenter; Scheidle, Heiko; Sidhu, Vishaldeep Kaur; Mrozek, Kalina et al.: Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases. In: BMC Microbiology 12 (2012), 239. DOI: https://doi.org/10.1186/1471-2180-12-239
dc.description.abstract Background: Efficient perception of attacking pathogens is essential for plants. Plant defense is evoked by molecules termed elicitors. Endogenous elicitors or damage-associated molecular patterns (DAMPs) originate from plant materials upon injury or pathogen activity. While there are comparably well-characterized examples for DAMPs, often oligogalacturonides (OGAs), generated by the activity of fungal pathogens, endogenous elicitors evoked by bacterial pathogens have been rarely described. In particular, the signal perception and transduction processes involved in DAMP generation are poorly characterized. Results: A mutant strain of the phytopathogenic bacterium Xanthomonas campestris pv. campestris deficient in exbD2, which encodes a component of its unusual elaborate TonB system, had impaired pectate lyase activity and caused no visible symptoms for defense on the non-host plant pepper (Capsicum annuum). A co-incubation of X. campestris pv. campestris with isolated cell wall material from C. annuum led to the release of compounds which induced an oxidative burst in cell suspension cultures of the non-host plant. Lipopolysaccharides and proteins were ruled out as elicitors by polymyxin B and heat treatment, respectively. After hydrolysis with trifluoroacetic acid and subsequent HPAE chromatography, the elicitor preparation contained galacturonic acid, the monosaccharide constituent of pectate. OGAs were isolated from this crude elicitor preparation by HPAEC and tested for their biological activity. While small OGAs were unable to induce an oxidative burst, the elicitor activity in cell suspension cultures of the non-host plants tobacco and pepper increased with the degree of polymerization (DP). Maximal elicitor activity was observed for DPs exceeding 8. In contrast to the X. campestris pv. campestris wild type B100, the exbD2 mutant was unable to generate elicitor activity from plant cell wall material or from pectin. Conclusions: To our knowledge, this is the second report on a DAMP generated by bacterial features. The generation of the OGA elicitor is embedded in a complex exchange of signals within the framework of the plant-microbe interaction of C. annuum and X. campestris pv. campestris. The bacterial TonB-system is essential for the substrate-induced generation of extracellular pectate lyase activity. This is the first demonstration that a TonB-system is involved in bacterial trans-envelope signaling in the context of a pathogenic interaction with a plant. eng
dc.description.sponsorship BMBF/GenoMik Plus
dc.language.iso eng
dc.publisher London : BioMed Central Ltd
dc.relation.ispartofseries BMC Microbiology 12 (2012)
dc.rights CC BY 2.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject TonB system eng
dc.subject Damage-associate molecular pattern eng
dc.subject DAMP eng
dc.subject Oligogalacturonide eng
dc.subject Trans-envelope signaling eng
dc.subject Molecular plant-microbe interaction eng
dc.subject Pathogen eng
dc.subject Xanthomonas campestris eng
dc.subject host-pathogen interactions eng
dc.subject elicits phytoalexin accumulation eng
dc.subject complete genome sequence eng
dc.subject f-sp glycinea eng
dc.subject hypersensitive response eng
dc.subject innate immunity eng
dc.subject disease resistance eng
dc.subject oxidative burst eng
dc.subject polygalacturonate lyase eng
dc.subject transduction systems eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Involvement of bacterial TonB-dependent signaling in the generation of an oligogalacturonide damage-associated molecular pattern from plant cell walls exposed to Xanthomonas campestris pv. campestris pectate lyases
dc.type Text
dc.type article
dc.relation.issn 1471-2180
dc.relation.doi https://doi.org/10.1186/1471-2180-12-239
dc.bibliographicCitation.volume 12
dc.bibliographicCitation.firstPage 239
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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