dc.identifier.uri |
http://dx.doi.org/10.15488/11791 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/11884 |
|
dc.contributor.author |
Alnajjar, Suhayla
|
|
dc.contributor.author |
Nolte, Ingo
|
|
dc.contributor.author |
Becker, Annegret
|
|
dc.contributor.author |
Schille, Jan Torben
|
|
dc.contributor.author |
Trakooljul, Nares
|
|
dc.contributor.author |
Frank, Marcus
|
|
dc.contributor.author |
Ngezahayo, Anaclet
|
|
dc.contributor.author |
Murua Escobar, Hugo
|
|
dc.date.accessioned |
2022-02-07T06:23:13Z |
|
dc.date.available |
2022-02-07T06:23:13Z |
|
dc.date.issued |
2021 |
|
dc.identifier.citation |
Alnajjar, S.; Nolte, I.; Becker, A.; Schille, J.T.; Trakooljul, N. et al.: Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention. In: International journal of molecular sciences 22 (2021), Nr. 22, 12289. DOI: https://doi.org/10.3390/ijms222212289 |
|
dc.description.abstract |
Claudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of Clostridium perfringens enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for prostate adenocarcinoma (PAC) and transitional cell carcinoma (TCC) cell killing in vitro using gold-nanoparticle-mediated laser perforation (GNOME-LP). A PAC and TCC cell lines, as well as red fluorescence variants, allowing deep tissue imaging, were used. CLDN-3, -4, and -7 expression was confirmed by qPCR and immunofluorescences. The binding of C-CPE-AuNPs complexes on the cell surface was examined by scanning electron microscopy (SEM). Further, transcriptome analysis was carried out to evaluate the effect of C-CPE binder on the biological response of treated cells. Directed C-CPE-AuNP binding verified the capability to target CLDN receptors. Transcriptome analysis showed that C-CPE binding may activate immune and inflammatory responses but does not directly affect cell survival. Cancer cells ablation was demonstrated using a combination of GNOME-LP and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10% depending on cell line. The fluorescent cell lines and the verified proof of concept in vitro provide the basis for perspective xenograft studies in an animal model. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Basel : Molecular Diversity Preservation International (MDPI) |
|
dc.relation.ispartofseries |
International journal of molecular sciences 22 (2021), Nr. 22 |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
cell lines |
eng |
dc.subject |
prostate cancer |
eng |
dc.subject |
C-CPE |
eng |
dc.subject |
gold nanoparticle |
eng |
dc.subject |
GNOME-LP |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1422-0067 |
|
dc.relation.doi |
10.3390/ijms222212289 |
|
dc.bibliographicCitation.issue |
22 |
|
dc.bibliographicCitation.volume |
22 |
|
dc.bibliographicCitation.firstPage |
12289 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|