dc.identifier.uri |
http://dx.doi.org/10.15488/11764 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/11857 |
|
dc.contributor.author |
Höfler, Simone
|
|
dc.contributor.author |
Lukat, Peer
|
|
dc.contributor.author |
Blankenfeldt, Wulf
|
|
dc.contributor.author |
Carlomagno, Teresa
|
|
dc.date.accessioned |
2022-02-03T07:45:36Z |
|
dc.date.available |
2022-02-03T07:45:36Z |
|
dc.date.issued |
2021 |
|
dc.identifier.citation |
Höfler, S.; Lukat, P.; Blankenfeldt, W.; Carlomagno, T.: Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites. In: Scientific reports 11 (2021), 17561. DOI: https://doi.org/10.1038/s41598-021-97030-y |
|
dc.description.abstract |
Box C/D ribonucleoprotein complexes are RNA-guided methyltransferases that methylate the ribose 2’-OH of RNA. The central ‘guide RNA’ has box C and D motifs at its ends, which are crucial for activity. Archaeal guide RNAs have a second box C’/D’ motif pair that is also essential for function. This second motif is poorly conserved in eukaryotes and its function is uncertain. Conflicting literature data report that eukaryotic box C’/D’ motifs do or do not bind proteins specialized to recognize box C/D-motifs and are or are not important for function. Despite this uncertainty, the architecture of eukaryotic 2’-O-methylation enzymes is thought to be similar to that of their archaeal counterpart. Here, we use biochemistry, X-ray crystallography and mutant analysis to demonstrate the absence of functional box C’/D’ motifs in more than 80% of yeast guide RNAs. We conclude that eukaryotic Box C/D RNPs have two non-symmetric protein assembly sites and that their three-dimensional architecture differs from that of archaeal 2’-O-methylation enzymes. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
[London] : Macmillan Publishers Limited, part of Springer Nature |
|
dc.relation.ispartofseries |
Scientific reports 11 (2021) |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Biochemistry |
eng |
dc.subject |
Molecular biology |
eng |
dc.subject |
Structural biology |
eng |
dc.subject.ddc |
500 | Naturwissenschaften
|
ger |
dc.subject.ddc |
600 | Technik
|
ger |
dc.title |
Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
2045-2322 |
|
dc.relation.doi |
10.1038/s41598-021-97030-y |
|
dc.bibliographicCitation.volume |
11 |
|
dc.bibliographicCitation.firstPage |
17561 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|