Abstract: | |
Key message: Symbiotic nitrogen fixation in root nodules of legumes is a highly important biological process which is only poorly understood. Root nodule metabolism differs from that of roots. Differences in root and nodule metabolism are expressed by altered protein abundances and amenable to quantitative proteome analyses. Differences in the proteomes may either be tissue specific and related to the presence of temporary endosymbionts (the bacteroids) or related to nitrogen fixation activity. An experimental setup including WT bacterial strains and strains not able to conduct symbiotic nitrogen fixation as well as root controls enables identification of tissue and nitrogen fixation specific proteins. Abstract: Root nodules are specialized plant organs housing and regulating the mutual symbiosis of legumes with nitrogen fixing rhizobia. As such, these organs fulfill unique functions in plant metabolism. Identifying the proteins required for the metabolic reactions of nitrogen fixation and those merely involved in sustaining the rhizobia:plant symbiosis, is a challenging task and requires an experimental setup which allows to differentiate between these two physiological processes. Here, quantitative proteome analyses of nitrogen fixing and non-nitrogen fixing nodules as well as fertilized and non-fertilized roots were performed using Vicia faba and Rhizobium leguminosarum. Pairwise comparisons revealed altered enzyme abundance between active and inactive nodules. Similarly, general differences between nodules and root tissue were observed. Together, these results allow distinguishing the proteins directly involved in nitrogen fixation from those related to nodulation. Further observations relate to the control of nodulation by hormones and provide supportive evidence for the previously reported correlation of nitrogen and sulfur fixation in these plant organs. Additionally, data on altered protein abundance relating to alanine metabolism imply that this amino acid may be exported from the symbiosomes of V. faba root nodules in addition to ammonia. Data are available via ProteomeXchange with identifier PXD008548. © 2018, Springer Science+Business Media B.V., part of Springer Nature.
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License of this version: | Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. |
Publication type: | Article |
Publishing status: | acceptedVersion |
Publication date: | 2018 |
Keywords english: | Cellular metabolism, Nodulation, Rhizobium leguminosarum, Shotgun mass spectrometry, Symbiotic nitrogen fixation, Vicia faba, plant protein, proteome, isolation and purification, liquid chromatography, metabolism, nitrogen fixation, nodulation, physiology, plant root, procedures, proteomics, Rhizobium leguminosarum, symbiosis, tandem mass spectrometry, Vicia faba, Chromatography, Liquid, Nitrogen Fixation, Plant Proteins, Plant Root Nodulation, Plant Roots, Proteome, Proteomics, Rhizobium leguminosarum, Root Nodules, Plant, Symbiosis, Tandem Mass Spectrometry, Vicia faba |
DDC: | 580 | Pflanzen (Botanik) |
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