Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Show simple item record

dc.identifier.uri Fondevilla, Sara Küster, Helge Krajinski, Franziska Cubero, José I. Rubiales, Diego 2017-02-23T13:12:47Z 2017-02-23T13:12:47Z 2011
dc.identifier.citation Fondevilla, S.; Küster, Helge; Krajinski, F.; Cubero, J.I.; Rubiales, D.: Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology. In: BMC Genomics 12 (2011), 28. DOI:
dc.description.abstract Background: Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in Pisum relatives. The identification of the genes underlying resistance would increase our knowledge about M. pinodes-pea interaction and would facilitate the introgression of resistance into pea varieties. In the present study differentially expressed genes in the resistant P. sativum ssp. syriacum accession P665 comparing to the susceptible pea cv. Messire after inoculation with M. pinodes have been identified using a M. truncatula microarray.Results: Of the 16,470 sequences analysed, 346 were differentially regulated. Differentially regulated genes belonged to almost all functional categories and included genes involved in defense such as genes involved in cell wall reinforcement, phenylpropanoid and phytoalexins metabolism, pathogenesis- related (PR) proteins and detoxification processes. Genes associated with jasmonic acid (JA) and ethylene signal transduction pathways were induced suggesting that the response to M. pinodes in pea is regulated via JA and ET pathways. Expression levels of ten differentially regulated genes were validated in inoculated and control plants using qRT-PCR showing that the P665 accession shows constitutively an increased expression of the defense related genes as peroxidases, disease resistance response protein 39 (DRR230-b), glutathione S-transferase (GST) and 6a-hydroxymaackiain methyltransferase.Conclusions: Through this study a global view of genes expressed during resistance to M. pinodes has been obtained, giving relevant information about the mechanisms and pathways conferring resistance to this important disease. In addition, the M. truncatula microarray represents an efficient tool to identify candidate genes controlling resistance to M. pinodes in pea. eng
dc.description.sponsorship Spanish Ministry of Science and Innovation
dc.description.sponsorship AGL2008-01239
dc.description.sponsorship FP6-CT-2004-FOOD-1-506223
dc.language.iso eng
dc.publisher London : BioMed Central Ltd.
dc.relation.ispartofseries BMC Genomics 12 (2011)
dc.rights CC BY 2.0 Unported
dc.subject glutathione transferase eng
dc.subject peroxidase eng
dc.subject article eng
dc.subject Ascomycetes eng
dc.subject cell wall eng
dc.subject controlled study eng
dc.subject gene expression eng
dc.subject gene expression regulation eng
dc.subject gene identification eng
dc.subject genetic association eng
dc.subject genetic resistance eng
dc.subject genetic susceptibility eng
dc.subject membrane transport eng
dc.subject microarray analysis eng
dc.subject Mycosphaerella pinodes eng
dc.subject nonhuman eng
dc.subject nucleotide sequence eng
dc.subject protein binding eng
dc.subject protein metabolism eng
dc.subject quantitative analysis eng
dc.subject reverse transcription polymerase chain reaction eng
dc.subject signal transduction eng
dc.subject gene expression profiling eng
dc.subject genetics eng
dc.subject immunology eng
dc.subject innate immunity eng
dc.subject methodology eng
dc.subject microbiology eng
dc.subject pea eng
dc.subject physiology eng
dc.subject plant disease eng
dc.subject Ascochyta eng
dc.subject Didymella pinodes eng
dc.subject Pisum eng
dc.subject Pisum sativum eng
dc.subject Ascomycota eng
dc.subject Gene Expression Profiling eng
dc.subject Immunity, Innate eng
dc.subject Microarray Analysis eng
dc.subject Peas eng
dc.subject Plant Diseases eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology
dc.type Article
dc.type Text
dc.relation.issn 1471-2164
dc.bibliographicCitation.volume 12
dc.bibliographicCitation.firstPage 28
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

Files in this item

This item appears in the following Collection(s):

Show simple item record


Search the repository


My Account

Usage Statistics