Analysis of Nucleosides and Nucleotides in Plants : An Update on Sample Preparation and LC–MS Techniques

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dc.identifier.uri http://dx.doi.org/10.15488/11203
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/11289
dc.contributor.author Straube, Henryk
dc.contributor.author Witte, Claus-Peter
dc.contributor.author Herde, Marco
dc.date.accessioned 2021-08-13T06:50:28Z
dc.date.available 2021-08-13T06:50:28Z
dc.date.issued 2021
dc.identifier.citation Straube, H.; Witte, C.-P.; Herde, M.: Analysis of Nucleosides and Nucleotides in Plants : An Update on Sample Preparation and LC–MS Techniques. In: Cells : open access journal 10 (2021), Nr. 3, 689. DOI: https://doi.org/10.3390/cells10030689
dc.description.abstract Nucleotides fulfill many essential functions in plants. Compared to non-plant systems, these hydrophilic metabolites have not been adequately investigated in plants, especially the less abundant nucleotide species such as deoxyribonucleotides and modified or damaged nucleotides. Until recently, this was mainly due to a lack of adequate methods for in-depth analysis of nucleotides and nucleosides in plants. In this review, we focus on the current state-of-the-art of nucleotide analysis in plants with liquid chromatography coupled to mass spectrometry and describe recent major advances. Tissue disruption, quenching, liquid–liquid and solid-phase extraction, chromatographic strategies, and peculiarities of nucleotides and nucleosides in mass spectrometry are covered. We describe how the different steps of the analytical workflow influence each other, highlight the specific challenges of nucleotide analysis, and outline promising future developments. The metabolite matrix of plants is particularly complex. Therefore, it is likely that nucleotide analysis methods that work for plants can be applied to other organisms as well. Although this review focuses on plants, we also discuss advances in nucleotide analysis from non-plant systems to provide an overview of the analytical techniques available for this challenging class of metabolites. eng
dc.language.iso eng
dc.publisher Basel : MDPI
dc.relation.ispartofseries Cells : open access journal 10 (2021), Nr. 3
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject nucleotides eng
dc.subject nucleosides eng
dc.subject mass spectrometry eng
dc.subject liquid chromatography eng
dc.subject quenching eng
dc.subject solid phase extraction eng
dc.subject hydrophilic interaction liquid chromatography (HILIC) eng
dc.subject plant nucleotide metabolism eng
dc.subject plant metabolomics eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Analysis of Nucleosides and Nucleotides in Plants : An Update on Sample Preparation and LC–MS Techniques
dc.type article
dc.type Text
dc.relation.essn 2073-4409
dc.relation.doi https://doi.org/10.3390/cells10030689
dc.bibliographicCitation.issue 3
dc.bibliographicCitation.volume 10
dc.bibliographicCitation.firstPage 689
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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