A highly mutable GST is essential for bract colouration in Euphorbia pulcherrima Willd. Ex Klotsch

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dc.identifier.uri http://dx.doi.org/10.15488/11175
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/11261
dc.contributor.author Vilperte, Vinicius
dc.contributor.author Boehm, Robert
dc.contributor.author Debener, Thomas
dc.date.accessioned 2021-08-12T11:25:52Z
dc.date.available 2021-08-12T11:25:52Z
dc.date.issued 2021
dc.identifier.citation Vilperte, V.; Boehm, R.; Debener, T.: A highly mutable GST is essential for bract colouration in Euphorbia pulcherrima Willd. Ex Klotsch. In: BMC Genomics 12 (2021), 208. DOI: https://doi.org/10.1186/s12864-021-07527-z
dc.description.abstract Background: Mutation breeding is an extraordinary tool in plant breeding to increase the genetic variability, where mutations in anthocyanin biosynthesis are targets to generate distinctive phenotypes in ornamental species. In poinsettia, ionizing radiation is routinely applied in breeding programs to obtaining a range of colours, with nearly all pink and white varieties being obtained after γ- or X-ray mutagenesis of red varieties. In the present study we performed a thorough characterization of a potential mutagenesis target gene as the main responsible for the ‘white paradox’ in poinsettia. Results: We identified a GST gene in poinsettia (Bract1) as an essential factor for the expression of anthocyanin-based red colouration of bracts, which presents a high phylogenetic similarity to known anthocyanin-related GSTs. Red poinsettia varieties and white mutants generated from these varieties by X-ray were analysed for polymorphisms related to the ‘white paradox’ in the species. A 4 bp mutation in a short repeat within the coding region of Bract1 is most likely responsible for the appearance of white phenotypes upon irradiation treatment. The polymorphism between wild-type and mutant alleles co-segregates with the phenotype in progeny from heterozygous red and white parents. Moreover, overexpression of Bract1 wild-type allele in Arabidopsis tt19 mutants restored the anthocyanin phenotype, while the Bract1 mutated allele showed to be non-functional. Conclusions: The identified repeat seems to be highly unstable, since mutated plants can be easily detected among fewer than 200 shoots derived from 10 mutated plants. Our data indicate that particular short repeat sequences, similar to microsatellite sequences or so-called dynamic mutations, might be hot spots for genetic variability. Moreover, the identification of the Bract1 mutation fills a gap on the understanding on the molecular mechanism of colour formation in poinsettia. eng
dc.language.iso eng
dc.publisher London : BioMed Central
dc.relation.ispartofseries BMC Genomics 12 (2021)
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Anthocyanin eng
dc.subject Euphorbia pulcherrima eng
dc.subject Ionizing radiation eng
dc.subject Glutathione S-transferase eng
dc.subject Mutation breeding eng
dc.subject Poinsettia eng
dc.subject Short repeat sequences eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title A highly mutable GST is essential for bract colouration in Euphorbia pulcherrima Willd. Ex Klotsch
dc.type article
dc.type Text
dc.relation.essn 1471-2164
dc.relation.doi https://doi.org/10.1186/s12864-021-07527-z
dc.bibliographicCitation.volume 12
dc.bibliographicCitation.firstPage 208
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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