dc.identifier.uri |
http://dx.doi.org/10.15488/11087 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/11169 |
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dc.contributor.author |
Aguilar Cascante, Francisco José
|
|
dc.contributor.author |
Ekramzadeh, Kimia
|
|
dc.contributor.author |
Scheper, Thomas
|
|
dc.contributor.author |
Beutel, Sascha
|
|
dc.date.accessioned |
2021-06-22T08:11:58Z |
|
dc.date.available |
2021-06-22T08:11:58Z |
|
dc.date.issued |
2020 |
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dc.identifier.citation |
Aguilar, F.; Ekramzadeh, K.; Scheper, T.; Beutel, S.: Whole-Cell Production of Patchouli Oil Sesquiterpenes in Escherichia coli: Metabolic Engineering and Fermentation Optimization in Solid–Liquid Phase Partitioning Cultivation. In: ACS Omega 5 (2020), Nr. 50, S. 32436-32446. DOI: https://doi.org/10.1021/acsomega.0c04590 |
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dc.description.abstract |
Patchouli oil is a major ingredient in perfumery, granting a dark-woody scent due to its main constituent (−)-patchoulol. The growing demand for patchouli oil has raised interest in the development of a biotechnological process to assure a reliable supply. Herein, we report the production of patchouli oil sesquiterpenes by metabolically engineered Escherichia coli strains, using solid–liquid phase partitioning cultivation. The (−)-patchoulol production was possible using the endogenous methylerythritol phosphate pathway and overexpressing a (−)-patchoulol synthase isoform from Pogostemon cablin but at low titers. To improve the (−)-patchoulol production, the exogenous mevalonate pathway was overexpressed in the multi-plasmid PTS + Mev strain, which increased the (−)-patchoulol titer 5-fold. Fermentation was improved further by evaluating several defined media, and optimizing the pH and temperature of culture broth, enhancing the (−)-patchoulol titer 3-fold. To augment the (−)-patchoulol recovery from fermentation, the solid–liquid phase partitioning cultivation was analyzed by screening polymeric adsorbers, where the Diaion HP20 adsorber demonstrated the highest (−)-patchoulol recovery from all tests. Fermentation was scaled-up to fed-batch bioreactors, reaching a (−)-patchoulol titer of 40.2 mg L–1 and productivity of 20.1 mg L–1 d–1. The terpene profile and aroma produced from the PTS + Mev strain were similar to the patchouli oil, comprising (−)-patchoulol as the main product, and α-bulnesene, trans-β-caryophyllene, β-patchoulene, and guaia-5,11-diene as side products. This investigation represents the first study of (−)-patchoulol production in E. coli by solid–liquid phase partitioning cultivation, which provides new insights for the development of sustainable bioprocesses for the microbial production of fragrant terpenes. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Washington, DC : ACS Publications |
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dc.relation.ispartofseries |
ACS Omega 5 (2020), Nr. 50 |
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dc.rights |
CC BY-NC-ND 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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dc.subject.ddc |
540 | Chemie
|
ger |
dc.subject.ddc |
660 | Technische Chemie
|
ger |
dc.title |
Whole-Cell Production of Patchouli Oil Sesquiterpenes in Escherichia coli: Metabolic Engineering and Fermentation Optimization in Solid–Liquid Phase Partitioning Cultivation |
|
dc.type |
Article |
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dc.type |
Text |
|
dc.relation.essn |
2470-1343 |
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dc.relation.doi |
https://doi.org/10.1021/acsomega.0c04590 |
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dc.bibliographicCitation.issue |
50 |
|
dc.bibliographicCitation.firstPage |
32436 |
|
dc.bibliographicCitation.lastPage |
32446 |
|
dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
|