Xeno-Free In Vitro Cultivation and Osteogenic Differentiation of hAD-MSCs on Resorbable 3D Printed RESOMER

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dc.identifier.uri http://dx.doi.org/10.15488/10932
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/11014
dc.contributor.author Kirsch, Marline
dc.contributor.author Herder, Annabelle-Christin
dc.contributor.author Boudot, Cécile
dc.contributor.author Karau, Andreas
dc.contributor.author Rach, Jessica
dc.contributor.author Handke, Wiebke
dc.contributor.author Seltsam, Axel
dc.contributor.author Scheper, Thomas
dc.contributor.author Lavrentieva, Antonina
dc.date.accessioned 2021-05-14T07:42:03Z
dc.date.available 2021-05-14T07:42:03Z
dc.date.issued 2020
dc.identifier.citation Kirsch, M.; Herder, A.-C.; Boudot, C.; Karau, A.; Rach, J. et al.: Xeno-Free In Vitro Cultivation and Osteogenic Differentiation of hAD-MSCs on Resorbable 3D Printed RESOMER®. In: Materials 13 (2020), Nr. 15, 3399. DOI: https://doi.org/10.3390/ma13153399
dc.description.abstract The development of alloplastic resorbable materials can revolutionize the field of implantation technology in regenerative medicine. Additional opportunities to colonize the three-dimensionally (3D) printed constructs with the patient’s own cells prior to implantation can improve the regeneration process but requires optimization of cultivation protocols. Human platelet lysate (hPL) has already proven to be a suitable replacement for fetal calf serum (FCS) in 2D and 3D cell cultures. In this study, we investigated the in vitro biocompatibility of the printed RESOMER® Filament LG D1.75 materials as well as the osteogenic differentiation of human mesenchymal stem cells (hMSCs) cultivated on 3D printed constructs under the influence of different medium supplements (FCS, human serum (HS) and hPL). Additionally, the in vitro degradation of the material was studied over six months. We demonstrated that LG D1.75 is biocompatible and has no in vitro cytotoxic effects on hMSCs. Furthermore, hMSCs grown on the constructs could be differentiated into osteoblasts, especially supported by supplementation with hPL. Over six months under physiological in vitro conditions, a distinct degradation was observed, which, however, had no influence on the biocompatibility of the material. Thus, the overall suitability of the material LG D1.75 to produce 3D printed, resorbable bone implants and the promising use of hPL in the xeno-free cultivation of human MSCs on such implants for autologous transplantation have been demonstrated. eng
dc.language.iso eng
dc.publisher Basel : MDPI
dc.relation.ispartofseries Materials 13 (2020), Nr. 15
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject resorbable polymers eng
dc.subject 3D printing eng
dc.subject in vitro biocompatibility eng
dc.subject RESOMER® eng
dc.subject in vitro degradation eng
dc.subject osteogenic differentiation eng
dc.subject human platelet lysate eng
dc.subject human serum eng
dc.subject fetal calve serum eng
dc.subject adipose tissue-derived mesenchymal stem cells (hAD-MSCs) eng
dc.subject.ddc 600 | Technik ger
dc.title Xeno-Free In Vitro Cultivation and Osteogenic Differentiation of hAD-MSCs on Resorbable 3D Printed RESOMER
dc.type Article
dc.type Text
dc.relation.essn 1996-1944
dc.relation.doi https://doi.org/10.3390/ma13153399
dc.bibliographicCitation.issue 15
dc.bibliographicCitation.volume 13
dc.bibliographicCitation.firstPage 3399
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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