Chemical Analysis of a “Miller-Type” Complex Prebiotic Broth: Part II: Gas, Oil, Water and the Oil/Water-Interface

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dc.identifier.uri Scherer, Sabrina Wollrab, Eva Codutti, Luca Carlomagno, Teresa da Costa, Stefan Gomes Volkmer, Andreas Bronja, Amela Schmitz, Oliver J. Ott, Albrecht 2017-01-27T08:36:55Z 2017-01-27T08:36:55Z 2016
dc.identifier.citation Scherer, S.; Wollrab, E.; Codutti, Luca; Carlomagno, T.; da, Costa, S.G. et al.: Chemical Analysis of a “Miller-Type” Complex Prebiotic Broth: Part II: Gas, Oil, Water and the Oil/Water-Interface. In: Origins of Life and Evolution of Biospheres 2016 (2016), S. 1-23. DOI:
dc.description.abstract We have analyzed the chemical variety obtained by Miller-Urey-type experiments using nuclear magnetic resonance (NMR) spectroscopy and coherent anti-Stokes Raman scattering (CARS) spectroscopy, gas chromatography followed by mass spectrometry (GC/MS) and two-dimensional gas chromatography followed by mass spectrometry (GCxGC/MS). In the course of a running Miller-Urey-type experiment, a hydrophobic organic layer emerged besides the hydrophilic aqueous phase and the gaseous phase that were initially present. The gas phase mainly consisted of aromatic compounds and molecules containing C≡C or C≡N triple bonds. The hydrophilic phase contained at least a few thousands of different molecules, primarily distributed in a range of 50 and 500 Da. The hydrophobic phase is characterized by carbon-rich, oil-like compounds and their amphiphilic derivatives containing oxygen with tensioactive properties. The presence of a wide range of oxidized molecules hints to the availability of oxygen radicals. We suggest that they intervene in the formation of alkylated polyethylene glycol (PEG) in the oil/water interface. CARS spectroscopy revealed distinct vibrational molecular signatures. In particular, characteristic spectral bands for cyanide compounds were observed if the broth was prepared with electric discharges in the gaseous phase. The characteristic spectral bands were absent if discharges were released onto the water surface. NMR spectroscopy on the same set of samples independently confirmed the observation. In addition, NMR spectroscopy revealed overall high chemical variability that suggests strong non-linearities due to interdependent, sequential reaction steps. © 2016 The Author(s) eng
dc.description.sponsorship Saarland University
dc.description.sponsorship HSFP
dc.description.sponsorship EU/FP7/HEALTH-F5-2008-200820
dc.language.iso eng
dc.publisher Dordrecht : Springer Netherlands
dc.relation.ispartofseries Origins of Life and Evolution of Biospheres 2016 (2016)
dc.rights CC BY 4.0 Unported
dc.subject coherent anti-Stokes Raman scattering (CARS) eng
dc.subject Complex chemical mixture eng
dc.subject GC/MS eng
dc.subject GCxGC/MS eng
dc.subject Miller-Urey experiment eng
dc.subject Molecular vibrations eng
dc.subject NMR eng
dc.subject Oil/water interface eng
dc.subject Origin of Life eng
dc.subject Phase-transfer-catalysis eng
dc.subject Radicals eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.subject.ddc 540 | Chemie ger
dc.title Chemical Analysis of a “Miller-Type” Complex Prebiotic Broth: Part II: Gas, Oil, Water and the Oil/Water-Interface eng
dc.type article
dc.type Text
dc.relation.essn 1573-0875
dc.relation.issn 0169-6149
dc.bibliographicCitation.volume 2016
dc.bibliographicCitation.firstPage 1
dc.bibliographicCitation.lastPage 23
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

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