Cell type-specific adhesion and migration on laser-structured opaque surfaces

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dc.identifier.uri http://dx.doi.org/10.15488/10704
dc.identifier.uri https://www.repo.uni-hannover.de/handle/123456789/10782
dc.contributor.author Schaeske, Jörn
dc.contributor.author Fadeeva, Elena
dc.contributor.author Schlie-Wolter, Sabrina
dc.contributor.author Deiwick, Andrea
dc.contributor.author Chichkov, Boris N.
dc.contributor.author Ingendoh-Tsakmakidis, Alexandra
dc.contributor.author Stiesch, Meike
dc.contributor.author Winkel, Andreas
dc.date.accessioned 2021-03-30T11:22:29Z
dc.date.available 2021-03-30T11:22:29Z
dc.date.issued 2020
dc.identifier.citation Schaeske, J.; Fadeeva, E.; Schlie-Wolter, S.; Deiwick, A.; Chichkov, B.N. et al.: Cell type-specific adhesion and migration on laser-structured opaque surfaces. In: International Journal of Molecular Sciences (IJMS) 21 (2020), Nr. 22, 8442. DOI: https://doi.org/10.3390/ijms21228442
dc.description.abstract Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. eng
dc.language.iso eng
dc.publisher Basel : Molecular Diversity Preservation International (MDPI)
dc.relation.ispartofseries International Journal of Molecular Sciences (IJMS) 21 (2020), Nr. 22
dc.rights CC BY 4.0 Unported
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject biomaterials eng
dc.subject cell exclusion assay eng
dc.subject cell proliferation eng
dc.subject cell spreading eng
dc.subject cytocompatibility eng
dc.subject focal adhesion eng
dc.subject in vitro screening eng
dc.subject primary vs. immortalized cell lines eng
dc.subject spike structures eng
dc.subject.ddc 570 | Biowissenschaften, Biologie ger
dc.title Cell type-specific adhesion and migration on laser-structured opaque surfaces
dc.type article
dc.type Text
dc.relation.essn 1422-0067
dc.relation.issn 1661-6596
dc.relation.doi https://doi.org/10.3390/ijms21228442
dc.bibliographicCitation.issue 22
dc.bibliographicCitation.volume 21
dc.bibliographicCitation.firstPage 8442
dc.description.version publishedVersion
tib.accessRights frei zug�nglich


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