Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair

Show simple item record

dc.identifier.uri Gniesmer, Sarah Brehm, Ralph Hoffmann, Andrea de Cassan, Dominik Menzel, Henning Hoheisel, Anna Lena Glasmacher, Birgit Willbold, Elmar Reifenrath, Janin Ludwig, Nils Zimmerer, Rüdiger Tavassol, Frank Gellrich, Nils-Claudius Kampmann, Andreas 2021-03-26T10:06:23Z 2021-03-26T10:06:23Z 2020
dc.identifier.citation Gniesmer, S.; Brehm, R.; Hoffmann, A.; de Cassan, D.; Menzel, H. et al.: Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair. In: PLoS ONE 15 (2020), Nr. 1, e0227563. DOI:
dc.description.abstract Rotator cuff tear is the most frequent tendon injury in the adult population. Despite current improvements in surgical techniques and the development of grafts, failure rates following tendon reconstruction remain high. New therapies, which aim to restore the topology and functionality of the interface between muscle, tendon and bone, are essentially required. One of the key factors for a successful incorporation of tissue engineered constructs is a rapid ingrowth of cells and tissues, which is dependent on a fast vascularization. The dorsal skinfold chamber model in female BALB/cJZtm mice allows the observation of microhemodynamic parameters in repeated measurements in vivo and therefore the description of the vascularization of different implant materials. In order to promote vascularization of implant material, we compared a porous polymer patch (a commercially available porous polyurethane based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fiber mats and chitosan-graft-PCL coated electrospun PCL (CS-g-PCL) fiber mats in vivo. Using intravital fluorescence microscopy microcirculatory parameters were analyzed repetitively over 14 days. Vascularization was significantly increased in CS-g-PCL fiber mats at day 14 compared to the porous polymer patch and uncoated PCL fiber mats. Furthermore CS-g-PCL fiber mats showed also a reduced activation of immune cells. Clinically, these are important findings as they indicate that the CS-g-PCL improves the formation of vascularized tissue and the ingrowth of cells into electrospun PCL scaffolds. Especially the combination of enhanced vascularization and the reduction in immune cell activation at the later time points of our study points to an improved clinical outcome after rotator cuff tear repair. © 2020 Gniesmer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. eng
dc.language.iso eng
dc.publisher San Francisco, CA : Public Library of Science (PLoS)
dc.relation.ispartofseries PLoS ONE 15 (2020), Nr. 1
dc.rights CC BY 4.0 Unported
dc.subject Vascularization eng
dc.subject biocompatibility eng
dc.subject rotator cuff tear eng
dc.subject CS-g-PCL eng
dc.subject.ddc 500 | Naturwissenschaften ger
dc.subject.ddc 610 | Medizin, Gesundheit ger
dc.title Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair
dc.type Article
dc.type Text
dc.relation.essn 1932-6203
dc.bibliographicCitation.issue 1
dc.bibliographicCitation.volume 15
dc.bibliographicCitation.firstPage e0227563
dc.description.version publishedVersion
tib.accessRights frei zug�nglich

Files in this item

This item appears in the following Collection(s):

Show simple item record


Search the repository


My Account

Usage Statistics