dc.identifier.uri |
http://dx.doi.org/10.15488/10628 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/10706 |
|
dc.contributor.author |
Schmitz, Carola
|
|
dc.contributor.author |
Pepelanova, Iliyana
|
|
dc.contributor.author |
Seliktar, Dror
|
|
dc.contributor.author |
Potekhina, Ekaterina
|
|
dc.contributor.author |
Belousov, Vsevolod V.
|
|
dc.contributor.author |
Scheper, Thomas
|
|
dc.contributor.author |
Lavrentieva, Antonina
|
|
dc.date.accessioned |
2021-03-26T08:44:47Z |
|
dc.date.available |
2021-03-26T08:44:47Z |
|
dc.date.issued |
2020 |
|
dc.identifier.citation |
Schmitz, C.; Pepelanova, I.; Seliktar, D.; Potekhina, E.; Belousov, V.V. et al.: Live reporting for hypoxia : Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters. In: Biotechnology and Bioengineering 117 (2020), Nr. 11, S. 3265-3276. DOI: https://doi.org/10.1002/bit.27503 |
|
dc.description.abstract |
Natural oxygen gradients occur in tissues of biological organisms and also in the context of three-dimensional (3D) in vitro cultivation. Oxygen diffusion limitation and metabolic oxygen consumption by embedded cells produce areas of hypoxia in the tissue/matrix. However, reliable systems to detect oxygen gradients and cellular response to hypoxia in 3D cell culture systems are still missing. In this study, we developed a system for visualization of oxygen gradients in 3D using human adipose tissue–derived mesenchymal stem cells (hAD-MSCs) modified to stably express a fluorescent genetically engineered hypoxia sensor HRE-dUnaG. Modified cells retained their stem cell characteristics in terms of proliferation and differentiation capacity. The hypoxia-reporter cells were evaluated by fluorescence microscopy and flow cytometry under variable oxygen levels (2.5%, 5%, and 7.5% O2). We demonstrated that reporter hAD-MSCs output is sensitive to different oxygen levels and displays fast decay kinetics after reoxygenation. Additionally, the reporter cells were encapsulated in bulk hydrogels with a variable cell number, to investigate the sensor response in model 3D cell culture applications. The use of hypoxia-reporting cells based on MSCs represents a valuable tool for approaching the genuine in vivo cellular microenvironment and will allow a better understanding of the regenerative potential of AD-MSCs. © 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC |
eng |
dc.language.iso |
eng |
|
dc.publisher |
New York, NY [u.a.] : Wiley |
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dc.relation.ispartofseries |
Biotechnology and Bioengineering 117 (2020), Nr. 11 |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
3D cell culture |
eng |
dc.subject |
AD-MSCs |
eng |
dc.subject |
hydrogels |
eng |
dc.subject |
hypoxia sensor |
eng |
dc.subject |
reporter cells |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.title |
Live reporting for hypoxia : Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1097-0290 |
|
dc.relation.issn |
0006-3592 |
|
dc.relation.issn |
0368-1467 |
|
dc.relation.doi |
https://doi.org/10.1002/bit.27503 |
|
dc.bibliographicCitation.issue |
11 |
|
dc.bibliographicCitation.volume |
117 |
|
dc.bibliographicCitation.firstPage |
3265 |
|
dc.bibliographicCitation.lastPage |
3276 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|