dc.identifier.uri |
http://dx.doi.org/10.15488/1043 |
|
dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/1067 |
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dc.contributor.author |
Friedrich, Steffen
|
|
dc.contributor.author |
Hemmerling, Franziska
|
|
dc.contributor.author |
Lindner, Frederick
|
|
dc.contributor.author |
Warnke, Anna
|
|
dc.contributor.author |
Wunderlich, Johannes
|
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dc.contributor.author |
Berkhan, Gesche
|
|
dc.contributor.author |
Hahn, Frank
|
|
dc.date.accessioned |
2017-01-12T09:06:26Z |
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dc.date.available |
2017-01-12T09:06:26Z |
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dc.date.issued |
2016 |
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dc.identifier.citation |
Friedrich, S.; Hemmerling, F.; Lindner, F.; Warnke, A.; Wunderlich, J. et al.: Characterisation of the broadly-specific O-methyl-transferase jerf from the late stages of jerangolid biosynthesis. In: Molecules 21 (2016), Nr. 11, 1443. DOI: http://dx.doi.org/10.3390/molecules21111443 |
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dc.description.abstract |
We describe the characterisation of the O-methyltransferase JerF from the late stages of jerangolid biosynthesis. JerF is the first known example of an enzyme that catalyses the formation of a non-aromatic, cyclic methylenolether. The enzyme was overexpressed in E. coli and the cell-free extracts were used in bioconversion experiments. Chemical synthesis gave access to a series of substrate surrogates that covered a broad structural space. Enzymatic assays revealed a broad substrate tolerance and high regioselectivity of JerF, which makes it an attractive candidate for an application in chemoenzymatic synthesis with particular usefulness for late stage application on 4-methoxy-5,6-dihydro-2H-pyran-2-one-containing natural products. © 2016 by the authors; licensee MDPI, Basel, Switzerland. |
eng |
dc.description.sponsorship |
DFG/HA 5841/2-1 |
|
dc.description.sponsorship |
DFG/Emmy Noether program |
|
dc.description.sponsorship |
EU/Marie Curie program |
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dc.description.sponsorship |
EU/293430 |
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dc.description.sponsorship |
Graduate School MINAS |
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dc.language.iso |
eng |
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dc.publisher |
Basel : MDPI AG |
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dc.relation.ispartofseries |
Molecules 21 (2016), Nr. 11 |
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dc.rights |
CC BY-NC-SA 4.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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dc.subject |
Chemoenzymatic synthesis |
eng |
dc.subject |
Chemoselectivity |
eng |
dc.subject |
Enzymes |
eng |
dc.subject |
Methylenolethers |
eng |
dc.subject |
Methyltransferases |
eng |
dc.subject |
Natural products |
eng |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Characterisation of the broadly-specific O-methyl-transferase jerf from the late stages of jerangolid biosynthesis |
eng |
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.issn |
1420-3049 |
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dc.relation.doi |
https://doi.org/10.3390/molecules21111443 |
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dc.bibliographicCitation.issue |
11 |
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dc.bibliographicCitation.volume |
21 |
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dc.bibliographicCitation.firstPage |
1443 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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