ForschungszentrenFrei zugängliche Publikationen aus den Forschungszentrenhttps://www.repo.uni-hannover.de/handle/123456789/122024-03-19T07:58:59Z2024-03-19T07:58:59ZTriple Modification of Alginate Hydrogels by Fibrin Blending, Iron Nanoparticle Embedding, and Serum Protein-Coating Synergistically Promotes Strong EndothelializationRichter, AlenaLi, YayaRehbock, ChristophBarcikowski, StephanHaverich, AxelWilhelmi, MathiasBöer, Ulrikehttps://www.repo.uni-hannover.de/handle/123456789/167652024-03-19T02:00:10Z2021-01-01T00:00:00ZTriple Modification of Alginate Hydrogels by Fibrin Blending, Iron Nanoparticle Embedding, and Serum Protein-Coating Synergistically Promotes Strong Endothelialization
Richter, Alena; Li, Yaya; Rehbock, Christoph; Barcikowski, Stephan; Haverich, Axel; Wilhelmi, Mathias; Böer, Ulrike
Stent therapy can reduce both morbidity and mortality of chronic coronary stenosis and acute myocardial infarction. However, delayed re-endothelialization, endothelial dysfunction, and chronic inflammation are still unsolved problems. Alginate hydrogels can be used as a coating for stent surfaces; however, complete and fast endothelialization cannot be achieved. In this study, alginate hydrogels are modified by fibrin blending, iron nanoparticle (Fe-NP) embedding, and serum protein coating (SPC) while surface properties and endothelialization capacity are monitored. Only a triple, synergetic modification of the alginate coating by simultaneous I) fibrin blending, II) Fe-NP addition complemented by III) SPC is found to significantly improve endothelial cell viability (live–dead-staining) and proliferation (WST-8 assay). These conditions yield formation of closed endothelial cell monolayers and an up to threefold increase (p < 0.01) in viability, while, interestingly, no effect is found when the modifications (I)–(III) are conducted individually. This synergetic effect is attributed to an accumulation of agglomerated Fe-NP and serum proteins along fibrin fibers, observed via laser scanning microscopy tracking nanoparticle scattering and tetramethylrhodamine (TRITC)-albumin fluorescence. These synergetic effects can pave the way toward a novel strategy for the modification of various hydrogel-based biomaterials and biomaterial coatings.
2021-01-01T00:00:00ZPublic involvement in the governance of population-level biomedical research: Unresolved questions and future directionsErikainen, SonjaFriesen, PhoebeRand, LeahJongsma, KarinDunn, MichaelSorbie, AnnieMcCoy, MatthewBell, JessicaBurgess, MichaelChen, HaidanChico, VickyCunningham-Burley, SarahDarbyshire, JulieDawson, RebeccaEvans, AndrewFahy, NickFinlay, TeresaFrith, LucyGoldenberg, AaronHinton, LisaHoppe, NilsHughes, NigelKoenig, BarbaraLignou, SapfoMcGowan, MichelleParker, MichaelPrainsack, BarbaraShabani, MahsaStaunton, CiaraThompson, RachelVarnai, KingaVayena, EffyWilliams, OliWilliamson, MaxChan, SarahSheehan, Markhttps://www.repo.uni-hannover.de/handle/123456789/167472024-03-17T13:52:36Z2020-01-01T00:00:00ZPublic involvement in the governance of population-level biomedical research: Unresolved questions and future directions
Erikainen, Sonja; Friesen, Phoebe; Rand, Leah; Jongsma, Karin; Dunn, Michael; Sorbie, Annie; McCoy, Matthew; Bell, Jessica; Burgess, Michael; Chen, Haidan; Chico, Vicky; Cunningham-Burley, Sarah; Darbyshire, Julie; Dawson, Rebecca; Evans, Andrew; Fahy, Nick; Finlay, Teresa; Frith, Lucy; Goldenberg, Aaron; Hinton, Lisa; Hoppe, Nils; Hughes, Nigel; Koenig, Barbara; Lignou, Sapfo; McGowan, Michelle; Parker, Michael; Prainsack, Barbara; Shabani, Mahsa; Staunton, Ciara; Thompson, Rachel; Varnai, Kinga; Vayena, Effy; Williams, Oli; Williamson, Max; Chan, Sarah; Sheehan, Mark
Population-level biomedical research offers new opportunities to improve population health, but also raises new challenges to traditional systems of research governance and ethical oversight. Partly in response to these challenges, various models of public involvement in research are being introduced. Yet, the ways in which public involvement should meet governance challenges are not well understood. We conducted a qualitative study with 36 experts and stakeholders using the World Café method to identify key governance challenges and explore how public involvement can meet these challenges. This brief report discusses four cross-cutting themes from the study: the need to move beyond individual consent; issues in benefit and data sharing; the challenge of delineating and understanding publics; and the goal of clarifying justifications for public involvement. The report aims to provide a starting point for making sense of the relationship between public involvement and the governance of population-level biomedical research, showing connections, potential solutions and issues arising at their intersection. We suggest that, in population-level biomedical research, there is a pressing need for a shift away from conventional governance frameworks focused on the individual and towards a focus on collectives, as well as to foreground ethical issues around social justice and develop ways to address cultural diversity, value pluralism and competing stakeholder interests. There are many unresolved questions around how this shift could be realised, but these unresolved questions should form the basis for developing justificatory accounts and frameworks for suitable collective models of public involvement in population-level biomedical research governance.
2020-01-01T00:00:00ZTopic space trajectories: A case study on machine learning literatureSchaefermeier, BastianStumme, GerdHanika, Tomhttps://www.repo.uni-hannover.de/handle/123456789/167442024-03-17T12:50:27Z2021-01-01T00:00:00ZTopic space trajectories: A case study on machine learning literature
Schaefermeier, Bastian; Stumme, Gerd; Hanika, Tom
The annual number of publications at scientific venues, for example, conferences and journals, is growing quickly. Hence, even for researchers it becomes harder and harder to keep track of research topics and their progress. In this task, researchers can be supported by automated publication analysis. Yet, many such methods result in uninterpretable, purely numerical representations. As an attempt to support human analysts, we present topic space trajectories, a structure that allows for the comprehensible tracking of research topics. We demonstrate how these trajectories can be interpreted based on eight different analysis approaches. To obtain comprehensible results, we employ non-negative matrix factorization as well as suitable visualization techniques. We show the applicability of our approach on a publication corpus spanning 50 years of machine learning research from 32 publication venues. In addition to a thorough introduction of our method, our focus is on an extensive analysis of the results we achieved. Our novel analysis method may be employed for paper classification, for the prediction of future research topics, and for the recommendation of fitting conferences and journals for submitting unpublished work. An advantage in these applications over previous methods lies in the good interpretability of the results obtained through our methods.
2021-01-01T00:00:00ZSelective Bacterial Targeting and Infection-Triggered Release of Antibiotic Colistin ConjugatesTegge, WernerGuerra, GiuliaHöltke, AlexanderSchiller, LauritzBeutling, UlrikeHarmrolfs, KirstenGröbe, LotharWullenkord, HannahXu, ChunfaWeich, HerbertBrönstrup, Markhttps://www.repo.uni-hannover.de/handle/123456789/167402024-03-17T20:47:19Z2021-01-01T00:00:00ZSelective Bacterial Targeting and Infection-Triggered Release of Antibiotic Colistin Conjugates
Tegge, Werner; Guerra, Giulia; Höltke, Alexander; Schiller, Lauritz; Beutling, Ulrike; Harmrolfs, Kirsten; Gröbe, Lothar; Wullenkord, Hannah; Xu, Chunfa; Weich, Herbert; Brönstrup, Mark
In order to render potent, but toxic antibiotics more selective, we have explored a novel conjugation strategy that includes drug accumulation followed by infection-triggered release of the drug. Bacterial targeting was achieved using a modified fragment of the human antimicrobial peptide ubiquicidin, as demonstrated by fluorophore-tagged variants. To limit the release of the effector colistin only to infection-related situations, we introduced a linker that was cleaved by neutrophil elastase (NE), an enzyme secreted by neutrophil granulocytes at infection sites. The linker carried an optimized sequence of amino acids that was required to assure sufficient cleavage efficiency. The antibacterial activity of five regioisomeric conjugates prepared by total synthesis was masked, but was released upon exposure to recombinant NE when the linker was attached to amino acids at the 1- or the 3-position of colistin. A proof-of-concept was achieved in co-cultures of primary human neutrophils and Escherichia coli that induced the secretion of NE, the release of free colistin, and an antibacterial efficacy that was equal to that of free colistin.
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