A DyP-type peroxidase of pleurotus sapidus with alkene cleaving activity

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Krahe, N.-K.; Berger, R.G.; Ersoy, F.: A DyP-type peroxidase of pleurotus sapidus with alkene cleaving activity. In: Molecules 25 (2020), Nr. 7, 1536. DOI: https://doi.org/10.3390/molecules25071536

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Alkene cleavage is a possibility to generate aldehydes with olfactory properties for the fragrance and flavor industry. A dye-decolorizing peroxidase (DyP) of the basidiomycete Pleurotus sapidus (PsaPOX) cleaved the aryl alkene trans-anethole. The PsaPOX was semi-purified from the mycelium via FPLC, and the corresponding gene was identified. The amino acid sequence as well as the predicted tertiary structure showed typical characteristics of DyPs as well as a non-canonical Mn2+-oxidation site on its surface. The gene was expressed in Komagataella pfaffii GS115 yielding activities up to 142 U/L using 2,20-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) as substrate. PsaPOX exhibited optima at pH 3.5 and 40 ◦C and showed highest peroxidase activity in the presence of 100 µM H2O2 and 25 mM Mn2+. PsaPOX lacked the typical activity of DyPs towards anthraquinone dyes, but oxidized Mn2+ to Mn3+. In addition, bleaching of β-carotene and annatto was observed. Biotransformation experiments verified the alkene cleavage activity towards the aryl alkenes (E)-methyl isoeugenol, α-methylstyrene, and trans-anethole, which was increased almost twofold in the presence of Mn2+. The resultant aldehydes are olfactants used in the fragrance and flavor industry. PsaPOX is the first described DyP with alkene cleavage activity towards aryl alkenes and showed potential as biocatalyst for flavor production.
License of this version: CC BY 4.0 Unported
Document Type: article
Issue Date: 2020
Appears in Collections:Naturwissenschaftliche Fakultät

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1 image of flag of Germany Germany 20 68.97%
2 image of flag of United States United States 7 24.14%
3 image of flag of Tanzania, United Republic of Tanzania, United Republic of 1 3.45%
4 image of flag of Russian Federation Russian Federation 1 3.45%

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