Small sample stress: Probing oxygen-deprived ammonia-oxidizing bacteria with raman spectroscopy in vivo

Download statistics - Document (COUNTER):

Kniggendorf, A.-K.; Nogueira, R.; Bahmanabad, S.N.; Pommerening-Röser, A.; Roth, B.W.: Small sample stress: Probing oxygen-deprived ammonia-oxidizing bacteria with raman spectroscopy in vivo. In: Microorganisms 8 (2020), Nr. 3, 432. DOI: https://doi.org/10.3390/microorganisms8030432

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/9891

Selected time period:

year: 
month: 

Sum total of downloads: 42




Thumbnail
Abstract: 
The stress response of ammonia-oxidizing bacteria (AOB) to oxygen deprivation limits AOB growth and leads to different nitrification pathways that cause the release of greenhouse gases. Measuring the stress response of AOB has proven to be a challenge due to the low growth rates of stressed AOB, making the sample volumes required to monitor the internal stress response of AOB prohibitive to repeated analysis. In a proof-of-concept study, confocal Raman microscopy with excitation resonant to the heme c moiety of cytochrome c was used to compare the cytochrome c content and activity of stressed and unstressed Nitrosomonas europaea (Nm 50), Nitrosomonas eutropha (Nm 57), Nitrosospira briensis (Nsp 10), and Nitrosospira sp. (Nsp 02) in vivo. Each analysis required no more than 1000 individual cells per sampling; thus, the monitoring of cultures with low cell concentrations was possible. The identified spectral marker delivered reproducible results within the signal-to-noise ratio of the underlying Raman spectra. Cytochrome c content was found to be elevated in oxygen-deprived and previously oxygen-deprived samples. In addition, cells with predominantly ferrous cytochrome c content were found in deprived Nitrosomonas eutropha and Nitrosospira samples, which may be indicative of ongoing electron storage at the time of measurement.
License of this version: CC BY 4.0 Unported
Document Type: article
Issue Date: 2020
Appears in Collections:Fakultät für Bauingenieurwesen und Geodäsie

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 29 69.05%
2 image of flag of China China 3 7.14%
3 image of flag of Norway Norway 2 4.76%
4 image of flag of Netherlands Netherlands 2 4.76%
5 image of flag of Indonesia Indonesia 2 4.76%
6 image of flag of United States United States 1 2.38%
7 image of flag of Russian Federation Russian Federation 1 2.38%
8 image of flag of Morocco Morocco 1 2.38%
9 image of flag of Austria Austria 1 2.38%

Further download figures and rankings:


Hinweis

Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository


Browse