Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines

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Sambale, F.; Wagner, S.; Stahl, F.; Khaydarov, R. R.; Scheper, T.; Bahnemann, Detlef W.: Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines. In: Journal of Nanomaterials 2015 (2015), 136765. DOI:

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Silver nanoparticles are widely used for many applications. In this study silver nanoparticles have been tested for their toxic effect on fibroblasts (NIH-3T3), on a human lung adenocarcinoma epithelial cell line (A-549), on PC-12-cells, a rat adrenal pheochromocytoma cell line, and on HEP-G2-cells, a human hepatocellular carcinoma cell line. The viability of the cells cultivated with different concentrations of silver was determined by the MTT assay, a photometric method to determine cell metabolism. Dose-response curves were extrapolated and IC50, total lethal concentration (TLC), and no observable adverse effect concentration (NOAEC) values were calculated for each cell line. As another approach, ECIS (electric-cell-substrate-impedance-sensing) an automated method to monitor cellular behavior in real-time was applied to observe cells cultivated with silver nanoparticles. To identify the type of cell death the membrane integrity was analyzed by measurements of the lactate dehydrogenase releases and by determination of the caspase 3/7 activity. To ensure that the cytotoxic effect of silver nanoparticles is not traced back to the presence of Ag+ ions in the suspension, an Ag+ salt (AgNO3) has been examined at the same concentration of Ag+ present in the silver nanoparticle suspension that is assuming that the Ag particles are completely available as Ag+ ions.
License of this version: CC BY 3.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2015
Appears in Collections:Naturwissenschaftliche Fakultät

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1 image of flag of Germany Germany 156 81.68%
2 image of flag of India India 7 3.66%
3 image of flag of China China 6 3.14%
4 image of flag of United States United States 3 1.57%
5 image of flag of Brazil Brazil 3 1.57%
6 image of flag of Singapore Singapore 2 1.05%
7 image of flag of Poland Poland 2 1.05%
8 image of flag of Indonesia Indonesia 2 1.05%
9 image of flag of France France 2 1.05%
10 image of flag of Anonymous Proxy Anonymous Proxy 2 1.05%
    other countries 6 3.14%

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