Cryopreservation of cells using defined serum-free cryoprotective agents

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Volbers, J.-C.; Lauterböck, L.; Hofmann, N.; Glasmacher, B.: Cryopreservation of cells using defined serum-free cryoprotective agents. In: Current Directions in Biomedical Engineering 2 (2016), Nr. 1, S. 315-318. DOI: https://doi.org/10.1515/cdbme-2016-0070

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Sum total of downloads: 117




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Abstract: 
For regenerative purposes, there is a high demand for viable and active cells. A big issue is to have enough viable cells available at any given time. One solution is cryopreservation. In this context, DMSO is used as cryoprotective agent (CPA) along with fetal bovine serum for nutrient supply and stress shielding effects. To use these cells for human clinical studies, it is important to eliminate the serum to prevent foreign immune reactions and virus transmittance and DMSO for its toxic effect. In this study a serum free cryopreservation solution and protocol has been established. The combination of methylcellulose and poloxamer 188 provide the basis for the new CPA. Other additves are -tocopherol, ectoine, prolin and ascorbic acid. The CPAs were examined with 3T3-cells and multipotent stromal cells from the common marmoset monkey (Callithrix jacchus). The cells were preserved with various CPA concentrations, incubation times and different cooling rates. To enable a higher throughput of encouraging conditions a fluorescence microscopy analysis was used. The use of methylcellulose, poloxamer 188 and -tocopherol enables the reduction of DMSO [up to 2.5% (v/v)] and the elimination of serum without viability losses compared to control.
License of this version: CC BY-NC-ND 4.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2016
Appears in Collections:Fakultät für Maschinenbau

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pos. country downloads
total perc.
1 image of flag of Germany Germany 57 48.72%
2 image of flag of United States United States 27 23.08%
3 image of flag of China China 5 4.27%
4 image of flag of Canada Canada 3 2.56%
5 image of flag of Austria Austria 3 2.56%
6 image of flag of No geo information available No geo information available 2 1.71%
7 image of flag of Thailand Thailand 2 1.71%
8 image of flag of Russian Federation Russian Federation 2 1.71%
9 image of flag of Iran, Islamic Republic of Iran, Islamic Republic of 2 1.71%
10 image of flag of Colombia Colombia 1 0.85%
    other countries 13 11.11%

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