Development of an aptamer-based affinity purification method for vascular endothelial growth factor

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Lönne, Maren; Bolten, Svenja Nicolin; Lavrentieva, Antonina; Stahl, Frank; Scheper, Thomas et al.: Development of an aptamer-based affinity purification method for vascular endothelial growth factor. In: Biotechnology Reports 8 (2015), S. 16-23. DOI: https://doi.org/10.1016/j.btre.2015.08.006

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Abstract: 
Since aptamers bind their targets with high affinity and specificity, they are promising alternative ligands in protein affinity purification. As aptamers are chemically synthesized oligonucleotides, they can be easily produced in large quantities regarding GMP conditions allowing their application in protein production for therapeutic purposes. Several advantages of aptamers compared to antibodies are described in general within this paper. Here, an aptamer directed against the human Vascular Endothelial Growth Factor (VEGF) was used as affinity ligand for establishing a purification platform for VEGF in small scale. The aptamer was covalently immobilized on magnetic beads in a controlled orientation resulting in a functional active affinity matrix. Target binding was optimized by introduction of spacer molecules and variation of aptamer density. Further, salt-induced target elution was demonstrated as well as VEGF purification from a complex protein mixture proving the specificity of protein-aptamer binding.
License of this version: CC BY-NC-ND 4.0
Document Type: article
Publishing status: publishedVersion
Issue Date: 2015
Appears in Collections:Naturwissenschaftliche Fakultät

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1 image of flag of Germany Germany 11 91.67%
2 image of flag of Spain Spain 1 8.33%

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