Quantification of microbial communities in subsurface marine sediments of the Black Sea and off Namibia

Download statistics - Document (COUNTER):

Schippers, Axel; Kock, Dagmar; Hoeft, Carmen; Koeweker, Gerrit; Siegert, Michael: Quantification of microbial communities in subsurface marine sediments of the Black Sea and off Namibia. In: Frontiers in Microbiology 3 (2012), 16. DOI: http://dx.doi.org/10.3389/fmicb.2012.00016

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/459

Selected time period:


Sum total of downloads: 246

Organic-rich subsurface marine sediments were taken by gravity coring up to a depth of 10 m below seafloor at six stations from the anoxic Black Sea and the Benguela upwelling system off Namibia during the research cruises Meteor 72-5 and 76-1, respectively. The quantitative microbial community composition at various sediment depths was analyzed using total cell counting, catalyzed reporter deposition fluorescence in situ hybridization (CARD FISH) and quantitative real-time PCR (Q-PCR). Total cell counts decreased with depths from 10(9) to 10(10) cells/mL at the sediment surface to 10(7)-10(9) cells/mL below one meter depth. Based on CARD FISH and Q-PCR analyses overall similar proportions of Bacteria and Archaea were found. The down-core distribution of prokaryotic and eukaryotic small subunit ribosomal RNA genes (16S and 18S rRNA) as well as functional genes involved in different biogeochemical processes was quantified using Q-PCR. Crenarchaeota and the bacterial candidate division JS-1 as well as the classes Anaerolineae and Caldilineae of the phylum Chloroflexi were highly abundant. Less abundant but detectable in most of the samples were Eukarya as well as the metal and sulfate-reducing Geobacteraceae (only in the Benguela upwelling influenced sediments). The functional genes cbbL, encoding for the large subunit of RuBisCO, the genes dsrA and aprA, indicative of sulfate-reducers as well as the mcrA gene of methanogens were detected in the Benguela upwelling and Black Sea sediments. Overall, the high organic carbon content of the sediments goes along with high cell counts and high gene copy numbers, as well as an equal abundance of Bacteria and Archaea.
License of this version: CC BY-NC 3.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2012
Appears in Collections:Naturwissenschaftliche Fakultät

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 187 76.02%
2 image of flag of United States United States 16 6.50%
3 image of flag of China China 13 5.28%
4 image of flag of France France 4 1.63%
5 image of flag of Switzerland Switzerland 4 1.63%
6 image of flag of No geo information available No geo information available 2 0.81%
7 image of flag of Russian Federation Russian Federation 2 0.81%
8 image of flag of Peru Peru 2 0.81%
9 image of flag of India India 2 0.81%
10 image of flag of Czech Republic Czech Republic 1 0.41%
    other countries 13 5.28%

Further download figures and rankings:


Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository