An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/ macrophages

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Beneke, V. et al.: An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. In: Scientific Reports (2018), Nr. 8, 16281. DOI: https://doi.org/:10.1038/s41598-018-34524-2

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To cite the version in the repository, please use this identifier: https://doi.org/10.15488/4728

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Sum total of downloads: 134




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Abstract: 
Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform.
License of this version: CC BY 4.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2018
Appears in Collections:Fakultät für Maschinenbau

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pos. country downloads
total perc.
1 image of flag of Germany Germany 91 67.91%
2 image of flag of United States United States 14 10.45%
3 image of flag of Czech Republic Czech Republic 8 5.97%
4 image of flag of Russian Federation Russian Federation 7 5.22%
5 image of flag of Vietnam Vietnam 3 2.24%
6 image of flag of India India 2 1.49%
7 image of flag of Finland Finland 2 1.49%
8 image of flag of Moldova, Republic of Moldova, Republic of 1 0.75%
9 image of flag of Japan Japan 1 0.75%
10 image of flag of Spain Spain 1 0.75%
    other countries 4 2.99%

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