A combined method for correlative 3D imaging of biological samples from macro to nano scale

Download statistics - Document (COUNTER):

Kellner, M.; Heidrich, M.; Lorbeer, R.-A.; Antonopoulos, G.C.; Knudsen, L. et al.: A combined method for correlative 3D imaging of biological samples from macro to nano scale. In: Scientific Reports 6 (2016), 35606. DOI: https://doi.org/10.1038/srep35606

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/4572

Selected time period:

year: 
month: 

Sum total of downloads: 46




Thumbnail
Abstract: 
Correlative analysis requires examination of a specimen from macro to nano scale as well as applicability of analytical methods ranging from morphological to molecular. Accomplishing this with one and the same sample is laborious at best, due to deformation and biodegradation during measurements or intermediary preparation steps. Furthermore, data alignment using differing imaging techniques turns out to be a complex task, which considerably complicates the interconnection of results. We present correlative imaging of the accessory rat lung lobe by combining a modified Scanning Laser Optical Tomography (SLOT) setup with a specially developed sample preparation method (CRISTAL). CRISTAL is a resin-based embedding method that optically clears the specimen while allowing sectioning and preventing degradation. We applied and correlated SLOT with Multi Photon Microscopy, histological and immunofluorescence analysis as well as Transmission Electron Microscopy, all in the same sample. Thus, combining CRISTAL with SLOT enables the correlative utilization of a vast variety of imaging techniques.
License of this version: CC BY 4.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2016
Appears in Collections:An-Institute

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 37 80.43%
2 image of flag of Netherlands Netherlands 3 6.52%
3 image of flag of United States United States 2 4.35%
4 image of flag of India India 1 2.17%
5 image of flag of Europe Europe 1 2.17%
6 image of flag of Estonia Estonia 1 2.17%
7 image of flag of Canada Canada 1 2.17%

Further download figures and rankings:


Hinweis

Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository


Browse