A combined method for correlative 3D imaging of biological samples from macro to nano scale

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Kellner, M.; Heidrich, M.; Lorbeer, R.-A.; Antonopoulos, G.C.; Knudsen, L. et al.: A combined method for correlative 3D imaging of biological samples from macro to nano scale. In: Scientific Reports 6 (2016), 35606. DOI: https://doi.org/10.1038/srep35606

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Sum total of downloads: 91

Correlative analysis requires examination of a specimen from macro to nano scale as well as applicability of analytical methods ranging from morphological to molecular. Accomplishing this with one and the same sample is laborious at best, due to deformation and biodegradation during measurements or intermediary preparation steps. Furthermore, data alignment using differing imaging techniques turns out to be a complex task, which considerably complicates the interconnection of results. We present correlative imaging of the accessory rat lung lobe by combining a modified Scanning Laser Optical Tomography (SLOT) setup with a specially developed sample preparation method (CRISTAL). CRISTAL is a resin-based embedding method that optically clears the specimen while allowing sectioning and preventing degradation. We applied and correlated SLOT with Multi Photon Microscopy, histological and immunofluorescence analysis as well as Transmission Electron Microscopy, all in the same sample. Thus, combining CRISTAL with SLOT enables the correlative utilization of a vast variety of imaging techniques.
License of this version: CC BY 4.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2016
Appears in Collections:An-Institute

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pos. country downloads
total perc.
1 image of flag of Germany Germany 61 67.03%
2 image of flag of United States United States 17 18.68%
3 image of flag of Netherlands Netherlands 3 3.30%
4 image of flag of Turkey Turkey 1 1.10%
5 image of flag of Sweden Sweden 1 1.10%
6 image of flag of Italy Italy 1 1.10%
7 image of flag of Iran, Islamic Republic of Iran, Islamic Republic of 1 1.10%
8 image of flag of India India 1 1.10%
9 image of flag of Europe Europe 1 1.10%
10 image of flag of Canada Canada 1 1.10%
    other countries 3 3.30%

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