Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway

Download statistics - Document (COUNTER):

Remelli, William; Guerrieri, Nicoletta; Klodmann, Jennifer; Papenbrock, Jutta; Pagani, Silvia; Forlani, Fabio: Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway. In: PLoS ONE 7 (2012), Nr. 9. DOI:

Repository version

To cite the version in the repository, please use this identifier:

Selected time period:


Sum total of downloads: 204

The phenotypic features of the Azotobacter vinelandii RhdA mutant MV474 (in which the rhdA gene was deleted) indicated that defects in antioxidant systems in this organism were related to the expression of the tandem-domain rhodanese RhdA. In this work, further insights on the effects of the oxidative imbalance generated by the absence of RhdA (e.g. increased levels of lipid hydroperoxides) are provided. Starting from the evidence that glutathione was depleted in MV474, and using both in silico and in vitro approaches, here we studied the interaction of wild-type RhdA and Cys(230) Ala site-directed RhdA mutant with glutathione species. We found that RhdA was able to bind in vitro reduced glutathione (GSH) and that RhdA-Cys(230) residue was mandatory for the complex formation. RhdA catalyzed glutathione-disulfide formation in the presence of a system generating the glutathione thiyl radical (GS(.), an oxidized form of GSH), thereby facilitating GSH regeneration. This reaction was negligible when the Cys230 Ala RhdA mutant was used. The efficiency of RhdA as catalyst in GS(.)-scavenging activity is discussed on the basis of the measured parameters of both interaction with glutathione species and kinetic studies.
License of this version: CC BY 3.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2012
Appears in Collections:Naturwissenschaftliche Fakultät

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 173 84.80%
2 image of flag of United States United States 8 3.92%
3 image of flag of China China 6 2.94%
4 image of flag of Brazil Brazil 4 1.96%
5 image of flag of No geo information available No geo information available 2 0.98%
6 image of flag of Russian Federation Russian Federation 2 0.98%
7 image of flag of France France 2 0.98%
8 image of flag of Israel Israel 1 0.49%
9 image of flag of Hungary Hungary 1 0.49%
10 image of flag of Europe Europe 1 0.49%
    other countries 4 1.96%

Further download figures and rankings:


Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository