Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway

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Remelli, William; Guerrieri, Nicoletta; Klodmann, Jennifer; Papenbrock, Jutta; Pagani, Silvia; Forlani, Fabio: Involvement of the Azotobacter vinelandii Rhodanese-Like Protein RhdA in the Glutathione Regeneration Pathway. In: PLoS ONE 7 (2012), Nr. 9. DOI: http://dx.doi.org/10.1371/journal.pone.0045193

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Abstract: 
The phenotypic features of the Azotobacter vinelandii RhdA mutant MV474 (in which the rhdA gene was deleted) indicated that defects in antioxidant systems in this organism were related to the expression of the tandem-domain rhodanese RhdA. In this work, further insights on the effects of the oxidative imbalance generated by the absence of RhdA (e.g. increased levels of lipid hydroperoxides) are provided. Starting from the evidence that glutathione was depleted in MV474, and using both in silico and in vitro approaches, here we studied the interaction of wild-type RhdA and Cys(230) Ala site-directed RhdA mutant with glutathione species. We found that RhdA was able to bind in vitro reduced glutathione (GSH) and that RhdA-Cys(230) residue was mandatory for the complex formation. RhdA catalyzed glutathione-disulfide formation in the presence of a system generating the glutathione thiyl radical (GS(.), an oxidized form of GSH), thereby facilitating GSH regeneration. This reaction was negligible when the Cys230 Ala RhdA mutant was used. The efficiency of RhdA as catalyst in GS(.)-scavenging activity is discussed on the basis of the measured parameters of both interaction with glutathione species and kinetic studies.
License of this version: CC BY 3.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2012
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 173 84.80%
2 image of flag of United States United States 8 3.92%
3 image of flag of China China 6 2.94%
4 image of flag of Brazil Brazil 4 1.96%
5 image of flag of No geo information available No geo information available 2 0.98%
6 image of flag of Russian Federation Russian Federation 2 0.98%
7 image of flag of France France 2 0.98%
8 image of flag of Israel Israel 1 0.49%
9 image of flag of Hungary Hungary 1 0.49%
10 image of flag of Europe Europe 1 0.49%
    other countries 4 1.96%

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