Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules

Download statistics - Document (COUNTER):

Limpens, Erik; Moling, Sjef; Hooiveld, Guido; Pereira, Patricia A.; Bisseling, Ton; et. al.: Cell- and Tissue-Specific Transcriptome Analyses of Medicago truncatula Root Nodules. In: PloS ONE 8 (2013), Nr. 5, e64377. DOI: http://dx.doi.org/10.1371/journal.pone.0064377

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/283

Selected time period:

year: 
month: 

Sum total of downloads: 167




Thumbnail
Abstract: 
Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ''. This digital "in situ'' offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies.
License of this version: CC BY 4.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2013-05-29
Appears in Collections:Naturwissenschaftliche Fakultät

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 153 91.62%
2 image of flag of United States United States 2 1.20%
3 image of flag of Paraguay Paraguay 1 0.60%
4 image of flag of Norway Norway 1 0.60%
5 image of flag of Netherlands Netherlands 1 0.60%
6 image of flag of Mexico Mexico 1 0.60%
7 image of flag of Kenya Kenya 1 0.60%
8 image of flag of India India 1 0.60%
9 image of flag of Croatia Croatia 1 0.60%
10 image of flag of France France 1 0.60%
    other countries 4 2.40%

Further download figures and rankings:


Hinweis

Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository


Browse