A peroxidase from Lepista irina cleaves beta,beta-carotene to flavor compounds

Download statistics - Document (COUNTER):

Zorn, Holger; Langhoff, S.; Scheibner, M.; Nimtz, M.; Berger, R. G.: A peroxidase from Lepista irina cleaves beta,beta-carotene to flavor compounds. In: Biological Chemistry 384 (2003), Nr. 7, S. 1049-1056. DOI: http://dx.doi.org/10.1515/BC.2003.117

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/239

Selected time period:

year: 
month: 

Sum total of downloads: 162




Thumbnail
Abstract: 
Extracellular liquid of the edible fungus Lepista irina was found to effectively degrade beta,beta-carotene. beta-Ionone, beta-cyclocitral, dihydroactinidiolide, and 2-hydroxy-2,6,6-trimethylcyclohexanone were formed as volatile breakdown products of beta,beta-carotene with myceliumfree culture supernatants, whereas beta-apo 10'-carotenal was identified as nonvolatile degradation product. The key enzyme catalyzing the oxidative cleavage of beta,beta-carotene was purified with an overall yield of 63% and a purification factor of 43. Biochemical characterization showed a molecular mass of 50.5 kDa and an isoelectric point of 3.75. Fastest beta,beta carotene degradation occurred at 34degreesC and pH values between 3.5 and 4. Degenerate oligonucleotides were derived from Nterminal and internal amino acid sequences. By means of PCRbased cDNAlibrary screening a 1284 bp cDNA was identified which showed great overall similarity to Pleurotus eryngii polyvalent peroxidases. The obtained sequence contains an open reading frame of 1083 nucleotides, encoding a polypeptide of 361 amino acids. A 30 amino acid signal peptide was identified upstream of the Nterminal sequence of the mature enzyme. The L. irina versatile peroxidase represents the first microbial enzyme capable of carotenoid degradation that has been characterized on a molecular level, proving the participation of extracellular enzymes of white rot fungi in biotic carotenoid degradation processes.
License of this version: Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Document Type: article
Publishing status: publishedVersion
Issue Date: 2003-07
Appears in Collections:Naturwissenschaftliche Fakultät

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 94 58.02%
2 image of flag of United States United States 9 5.56%
3 image of flag of Japan Japan 8 4.94%
4 image of flag of France France 6 3.70%
5 image of flag of China China 6 3.70%
6 image of flag of Netherlands Netherlands 4 2.47%
7 image of flag of Pakistan Pakistan 3 1.85%
8 image of flag of India India 3 1.85%
9 image of flag of United Kingdom United Kingdom 2 1.23%
10 image of flag of Brazil Brazil 2 1.23%
    other countries 25 15.43%

Further download figures and rankings:


Hinweis

Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository


Browse