The record length and quality of instantaneous peak flows (IPFs) have a great influence on flood design, but these high resolution flow data are not always available. The primary aim of this study is to compare different strategies to derive frequency distributions of IPFs using the Hydrologiska Byråns Vattenbalansavdelning (HBV) hydrologic model. The model is operated on a daily and an hourly time step for 18 catchments in the Aller-Leine basin, Germany. Subsequently, general extreme value (GEV) distributions are fitted to the simulated annual series of daily and hourly extreme flows. The resulting maximum mean daily flow (MDF) quantiles from daily simulations are transferred into IPF quantiles using a multiple regression model, which enables a direct comparison with the simulated hourly quantiles. As long climate records with a high temporal resolution are not available, the hourly simulations require a disaggregation of the daily rainfall. Additionally, two calibrations strategies are applied: (1) a calibration on flow statistics; (2) a calibration on hydrographs. The results show that: (1) the multiple regression model is capable of predicting IPFs with the simulated MDFs; (2) both daily simulations with post-correction of flows and hourly simulations with pre-processing of precipitation enable a reasonable estimation of IPFs; (3) the best results are achieved using disaggregated rainfall for hourly modelling with calibration on flow statistics; and (4) if the IPF observations are not sufficient for model calibration on flow statistics, the transfer of MDFs via multiple regressions is a good alternative for estimating IPFs. This is the peer reviewed version of the following article: Ding, J.; Wallner, M.; Müller, H.; Haberlandt, U.: Estimation of instantaneous peak flows using HMB hydrological model. In: Hydrological Processes 30 (2016), S.1431-1448, which has been published in final form at https://doi.org/10.1002/hyp.10725. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.