Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

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Song, Zhongshu; Bakeer, Walid; Marshall, James W.; Yakasai, Ahmed A.; Khalid, Rozida Mohd; Collemare, Jerome; Skellam, Elizabeth; Tharreau, Didier; Lebrun, Marc-Henri; Lazarus, Colin M.; Bailey, Andrew M.; Simpson, Thomas J.; Cox, Russell J.: Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. In: Chemical Science 6 (2015), Nr. 8, S. 4837-4845. DOI: http://dx.doi.org/10.1039/c4sc03707c

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Abstract: 
The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.
License of this version: CC BY 3.0 Unported
Document Type: article
Publishing status: publishedVersion
Issue Date: 2015-06-01
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 173 86.07%
2 image of flag of United States United States 12 5.97%
3 image of flag of China China 5 2.49%
4 image of flag of Malaysia Malaysia 1 0.50%
5 image of flag of Kenya Kenya 1 0.50%
6 image of flag of Japan Japan 1 0.50%
7 image of flag of Iran, Islamic Republic of Iran, Islamic Republic of 1 0.50%
8 image of flag of Indonesia Indonesia 1 0.50%
9 image of flag of France France 1 0.50%
10 image of flag of Belgium Belgium 1 0.50%
    other countries 4 1.99%

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