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Download statistics - Document (COUNTER):
Glyk, A.; Heinisch, S.L.; Scheper, T.; Beutel, S.: Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems. In: Analytical Biochemistry 477 (2015), S. 35-37. DOI: https://doi.org/10.1016/j.ab.2015.02.007
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Sum total of downloads: 433
| Abstract: | |
In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above 1 and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is favored in comparison with the Bradford assay. | |
| License of this version: | CC BY-NC-ND 4.0 Unported |
| Document Type: | Article |
| Publishing status: | acceptedVersion |
| Issue Date: | 2015 |
| Appears in Collections: | Naturwissenschaftliche Fakultät |
distribution of downloads over the selected time period:
downloads by country:
| pos. | country | downloads | ||
|---|---|---|---|---|
| total | perc. | |||
| 1 |  |
Germany | 210 | 48.50% |
| 2 |  |
United States | 61 | 14.09% |
| 3 |  |
United Kingdom | 24 | 5.54% |
| 4 |  |
China | 20 | 4.62% |
| 5 |  |
Canada | 10 | 2.31% |
| 6 |  |
Turkey | 9 | 2.08% |
| 7 |  |
Mexico | 8 | 1.85% |
| 8 |  |
Sweden | 7 | 1.62% |
| 9 |  |
India | 7 | 1.62% |
| 10 |  |
Australia | 7 | 1.62% |
| other countries | 70 | 16.17% | ||
Further download figures and rankings:
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